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PXD030043-1

PXD030043 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleQuantitative analysis of redox proteome reveals protein thiol switches regulating fundamental processes in developmental haematopoiesis
DescriptionFoetal and adult hematopoietic stem and progenitor cells (HSPCs) are characterized by distinct redox homeostasis that may influence their differential cellular behaviour in normal and malignant haematopoiesis. In this work, we have applied a quantitative mass spectrometry-based redox proteomic approach to comprehensively describe reversible cysteine modifications in primary mouse foetal and adult HSPCs. We defined the redox state of 4455 cysteines in foetal and adult HSPCs and demonstrated a higher susceptibility to oxidation of protein thiols in foetal HSPCs. Our data identified ontogenically active redox switches in proteins with a pronounced role in metabolism and protein homeostasis. Additional redox proteomic analysis identified redox switches acting in mitochondrial respiration as well as protein homeostasis to be triggered during onset of MLL-ENL leukemogenesis in foetal HSPCs. Our data has demonstrated that redox signalling contributes to the regulation of fundamental processes of developmental haematopoiesis and has pinpointed potential targetable redox-sensitive proteins in in utero-initiated MLL-rearranged leukaemia.
HostingRepositoryPRIDE
AnnounceDate2022-06-09
AnnouncementXMLSubmission_2022-06-09_05:25:21.579.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterKristyna Pimkova
SpeciesList scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationListacetylated residue; monohydroxylated residue
InstrumentOrbitrap Fusion
Dataset History
RevisionDatetimeStatusChangeLog Entry
02021-11-29 08:22:38ID requested
12022-06-09 05:25:22announced
Publication List
Pimkova K, Jassinskaja M, Munita R, Ciesla M, Guzzi N, Cao Thi Ngoc P, Vajrychova M, Johansson E, Bellodi C, Hansson J, Quantitative analysis of redox proteome reveals oxidation-sensitive protein thiols acting in fundamental processes of developmental hematopoiesis. Redox Biol, 53():102343(2022) [pubmed]
Keyword List
submitter keyword: hematopoiesis, leukemia, redox proteomics, oxidative modifications
Contact List
Kristyna Pimkova
contact affiliationLund Stem Cell Center, Division of Molecular Hematology, Lund University, Lund, Sweden
contact emailkristyna.pimkova@lf1.cuni.cz
lab head
Kristyna Pimkova
contact affiliationBIOCEV, 1st Medical Faculty, Charles University, Vestec, Czech Republic
contact emailkristyna.pimkova@lf1.cuni.cz
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
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