PXD030029-1

PXD030029 is an original dataset announced via ProteomeXchange.

Title	Quantitative Proteomics Analysis reveals Proteins related with high melatonin content in barley seeds under NaCl-induced salt stress
Description	Increase soil salinization is affecting hull-less barley cultivation in the Qinghai-Tibet Plateau of China. Under saline conditions wild hull-less barley seeds showed high melatonin (MEL) accumulation during germination with improve salt tolerance. Hence, the present study aimed to understand the proteome level changes that result in high melatonin content in the germinating hull-less barley seed after treatment with 240mM-NaCl concentrations compared to control. Our results indicate that salt stress induced global changes in the proteome of germinating barley seeds. The NaCl treatment altered the expression and abundance of several proteins related to carbohydrate and energy metabolism, amino acid transport and metabolism which contributed to the high melatonin content in the seeds under salt stress. Further, proteins associated with cellular redox homeostasis, osmotic stress response and secondary metabolites derived from amino acid metabolism, including purine degradation, and shikimate pathways were induced in seeds with high melatonin content. Consequently, triggering a robust response to the oxidative stress occasioned by the NaCl-induced salt stress and lead to improved germination rate and salt stress adaptation in hull-less barley seeds. Overall our finding contribute to our understanding of the mechanism of melatonin mediated salt stress tolerance in barley at the protein level.
HostingRepository	iProX
AnnounceDate	2021-11-29
AnnouncementXML	Submission_2023-12-21_22:14:29.437.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Guoqiang Zhang
SpeciesList	scientific name: Hordeum vulgare; NCBI TaxID: 4513;
ModificationList	TMT6plex reporter fragment
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2021-11-28 22:15:52	ID requested
⏵ 1	2023-12-21 22:14:30	announced

Zhang G, Yan Y, Zeng X, Wang Y, Zhang Y, Quantitative Proteomics Analysis Reveals Proteins Associated with High Melatonin Content in Barley Seeds under NaCl-Induced Salt Stress. J Agric Food Chem, 70(27):8492-8510(2022) [pubmed]

submitter keyword: Melatonin, TMT-based quantitative proteomics, Hull-less barley, NaCl, Salt stress, Amino acids, Seed germination, Secondary metabolites

Guoqiang Zhang
contact affiliation	Anhui Polytechnic University
contact email	zhangguoqiang@ahpu.edu.cn
lab head
Guoqiang Zhang
contact affiliation	Anhui Polytechnic University
contact email	zhangguoqiang@ahpu.edu.cn
dataset submitter

iProX dataset URI