PXD030027-1
PXD030027 is an original dataset announced via ProteomeXchange.
Dataset Summary
Title | An antibody-free enrichment approach of monomethyllysine peptides base on reductive glutaraldehydation and hydrazide chemistry |
Description | Protein lysine monomethylation is an important post-translational modification participated in regulating many biological processes thus there is growing interest in identifying these methylation events. However, the introduction of one methyl group on lysine residues has negligible effect on changing the physical and chemical properties of proteins or peptides, making enriching and identifying monomethylated lysine (Kme1) proteins or peptides extraordinarily challenging. In this study, we developed an antibody-free chemical proteomics approach to effectively capture Kme1 peptides, involving reductive glutaraldehydation of peptides and hydrazide chemistry. By optimizing the reaction condition of reductive glutaraldehydation, aldehyde modified Kme1 residues and piperidine modified primary amines were generated at the same time so that solid-phase hydrazide chemistry could be applied to effectively capture Kme1 peptides from complex mixture. Using this chemical approach, we can enrich and detect Kme1 peptide from peptide mixture containing 5000-fold bovine serum albumin tryptic digest. |
HostingRepository | iProX |
AnnounceDate | 2021-11-26 |
AnnouncementXML | Submission_2021-11-28_22:10:52.627.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Zhouxian Li |
SpeciesList | scientific name: Homo sapiens; NCBI TaxID: 9606; |
ModificationList | monomethylated residue |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
---|---|---|---|
0 | 2021-11-28 22:10:29 | ID requested | |
⏵ 1 | 2021-11-28 22:10:53 | announced |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: enrichment of monomethyllysine peptides, reductive glutaraldehydation, hydrazide chemistry |
Contact List
Weibing Zhang | |
---|---|
contact affiliation | East China University of Science and Technology |
contact email | weibingzhang@ecust.edu.cn |
lab head | |
Zhouxian Li | |
contact affiliation | East China University of Science and Technology |
contact email | lizhouxian17@163.com |
dataset submitter |
Full Dataset Link List
iProX dataset URI |