PXD029980-1

PXD029980 is an original dataset announced via ProteomeXchange.

Title	Mice tPVAT proteome profiling using high-throughput comparative proteomic analysis based on TMT labeling
Description	High-throughput comparative proteomic analysis based on TMT labeling combined with LC-MS/MS were performed on an in vivo Ang II infusion mice model to obtain a comprehensive view of the protein ensembles associated with thoracic PVAT (tPVAT) dysfunction induced by Ang II. In total, 5,037 proteins were confidently identified, of which 4,984 proteins were quantified. Compared with saline group, 145 proteins were upregulated and 146 proteins were downregulated during Ang II-induced tPVAT pathogenesis. Bioinformatics analyses revealed that the most enriched GO terms were annotated as gene silencing, monosaccharide binding, and extracellular matrix. In addition, some novel proteins potentially associated with Ang II infusion were identified, such as acyl-CoA carboxylase α, very long-chain acyl-CoA synthetase (ACSVL), uncoupling protein 1 (UCP1), perilipin, RAS protein-specific guanine nucleotide-releasing factor 2 (RasGRF2), and hypoxia inducible factor 1α (HIF-1α). Ang II could directly participate in the regulation of lipid metabolism, transportation, and adipocyte differentiation by affecting UCP1 and perilipin. Importantly, the key KEGG pathways were involved in fatty acid biosynthesis, FABP3-PPARα/γ, RasGRF2-ERK-HIF-1α, RasGRF2-PKC-HIF-1α, and STAT3-HIF-1α axis.
HostingRepository	PRIDE
AnnounceDate	2022-02-17
AnnouncementXML	Submission_2022-02-17_06:06:58.288.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Wenjuan Yao
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2021-11-26 08:09:44	ID requested
⏵ 1	2022-02-17 06:06:59	announced

Liang X, Guan H, Sun J, Qi Y, Yao W, Comparative Proteomic Analysis of tPVAT during Ang II Infusion. Biomedicines, 9(12):(2021) [pubmed]

submitter keyword: Mice, Ang II, tPVAT, LC-MSMS

Wenjuan Yao
contact affiliation	Nantong University
contact email	juanwenyy@ntu.edu.cn
lab head
Wenjuan Yao
contact affiliation	Nantong University
contact email	juanwenyy@ntu.edu.cn
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/02/PXD029980

PRIDE project URI

• PRIDE
  1. PXD029980
     1. Label: PRIDE project
     2. Name: Mice tPVAT proteome profiling using high-throughput comparative proteomic analysis based on TMT labeling