PXD029924-3

PXD029924 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	KOPI - Kinase inhibitor proteome impact analysis
Description	Kinase inhibitors often exhibit on/off-target effects, and efficient data analysis is essential for assessing these effects on the proteome. We developed a workflow for rapidly performing such a proteomic assessment, termed as kinase inhibitor proteome impact analysis (KOPI). We demonstrate KOPI’s utility with staurosporine on the leukemic K562 cell proteome. We analysed the changes in the proteome, thermal stability and phosphoproteome in the cells response to staurosporine. We identified systemically staurosporine’s non-kinome interactors, and showed for the first time that it caused paradoxical hyper- and biphasic phosphorylation.
HostingRepository	jPOST
AnnounceDate	2022-06-16
AnnouncementXML	Submission_2023-04-02_20:19:10.180.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Radoslaw Sobota
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	S-carboxamidomethyl-L-cysteine; L-methionine sulfoxide; O-phospho-L-serine; O-phospho-L-threonine; O4'-phospho-L-tyrosine; alpha-amino acetylated residue; deamidated L-glutamine; deamidated L-asparagine
Instrument	instrument

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2021-11-26 01:31:52	ID requested
1	2022-06-16 06:31:38	announced
2	2022-09-18 02:36:10	announced	2022-09-18: Updated FTP location.
⏵ 3	2023-04-02 20:19:11	announced	2023-04-03: Updated PubMed.

Publication List

Li GX, Zhao T, Wang LC, Choi H, Lim YT, Sobota RM, KOPI: Kinase inhibitOr Proteome Impact analysis. Sci Rep, 12(1):13015(2022) [pubmed]

Li GX, Zhao T, Wang LC, Choi H, Lim YT, Sobota RM, KOPI: Kinase inhibitOr Proteome Impact analysis. Sci Rep, 12(1):13015(2022) [pubmed]

Keyword List

submitter keyword: TMT, CETSA, protein expression, phosphorylation

submitter keyword: TMT, CETSA, protein expression, phosphorylation

Contact List

Lim Yan Ting, Radoslaw Sobota
lab head
Radoslaw Sobota
contact affiliation	IMCB A-STAR Singapore
dataset submitter

Full Dataset Link List

jPOST dataset URI
Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST001389/

jPOST dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST001389/