PXD029900 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Proteome dataset of cultured human bone marrow derived stromal (stem) cells, adipocytes and osteoblasts |
Description | Mesenchymal stromal stem cells (MSCs) gain increasing focus in the field of regenerative medicine due to their differentiational abilities into chondrocytes, adipocytes and osteoblastic cells. However, it is apparent that the transformation processes are extremely complex, cause cellular heterogeneity and need further insights into functional annotation. The aim of the study was to characterize differences between MSCs and cells after adipogenic or osteoblastic differentiation at the proteome level. For this, we analysed the proteome of isolated and cultured MSCs and after differentiation into adipogenic and osteoblastic cell lineages using electrospray ionization tandem mass spectrometry in data-independent acquisition mode. Protein inference and quantification was performed using deep neural networks in library free mode. By jointly using strict differential expression analysis and machine learning algorithms, two cell type specific marker protein panels for the osteoblastic and the adipocytic lineage were identified which allow for distinguishment of the three cell types. |
HostingRepository | PRIDE |
AnnounceDate | 2022-03-25 |
AnnouncementXML | Submission_2022-03-25_02:30:32.687.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Thorben Sauer |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | No PTMs are included in the dataset |
Instrument | TripleTOF 5600 |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2021-11-24 06:22:58 | ID requested | |
⏵ 1 | 2022-03-25 02:30:33 | announced | |
2 | 2022-03-25 08:57:09 | announced | 2022-03-25: Updated publication reference for PubMed record(s): 35269711. |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: LC-MSMS, DIA, Human |
Contact List
Timo Gemoll |
contact affiliation | Section for Translational Surgical Oncology and Biobanking, Department of Surgery, University of Lübeck and University Hospital Schleswig-Holstein, Campus Lübeck, Germany |
contact email | timo.gemoll@uni-luebeck.de |
lab head | |
Thorben Sauer |
contact affiliation | Section for translational surgical oncology and biobanking |
contact email | thorben.sauer@student.uni-luebeck.de |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/03/PXD029900 |
PRIDE project URI |
Repository Record List
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[ - ]
- PRIDE
- PXD029900
- Label: PRIDE project
- Name: Proteome dataset of cultured human bone marrow derived stromal (stem) cells, adipocytes and osteoblasts