PXD029794-2

PXD029794 is an original dataset announced via ProteomeXchange.

Title	Profiling of protein interactions of the circRNA cZNF292 in endothelial cells
Description	Circular RNAs are a class of non-coding RNAs mostly generated by back-splicing RNA transcripts of protein-coding gene loci. In difference to canoncial splicing, back-splicing ligates downstream splice donor site with an upstream splice acceptor generating a covalently closed circular RNA. CircRNAs have been reported to influence cellular functions by acting as miRNA sponges, regulation of host gene transcription or by protein interactions. The circRNA cZNF292 has been previously reported to be regulated under hypoxia and to influence endothelial cell angiogenesis and functions. The present data set aims to identfy protein interactions partners of cZNF292 in endothelial cells to further characterize the mechanism of action of cZNF292. Dataset comprises 8 replicates of proteins identified after biotin-based 2'O-Me RNA antisense affinity purification of the circRNA cZNF292 from HUVEC (human umbilical vein endothelial cell) lysates compared to a control probe dataset. Purification was performed without cross-linking under low salt and mild detergent conditions.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_07:22:31.896.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Ilka Wittig
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2021-11-18 00:00:41	ID requested
1	2022-11-29 01:51:33	announced
⏵ 2	2023-11-14 07:22:32	announced	2023-11-14: Updated project metadata.

Heum, ü, ller AW, Jones AN, Mour, ã, o A, Klangwart M, Shi C, Wittig I, Fischer A, Muhly-Reinholz M, Buchmann GK, Dieterich C, Potente M, Braun T, Grote P, Ja, é N, Sattler M, Dimmeler S, Locus-Conserved Circular RNA cZNF292 Controls Endothelial Cell Flow Responses. Circ Res, 130(1):67-79(2022) [pubmed]

submitter keyword: affinity purification, endothelial cells,LC-MSMS, circRNA

Stefanie Dimmeler
contact affiliation	Institute of Cardiovascular Regeneration, Goethe University, Frankfurt am Main, Germany
contact email	dimmeler@em.uni-frankfurt.de
lab head
Ilka Wittig
contact affiliation	Functional Proteomics, Goethe University, Frankfurt am Main , Germany
contact email	wittig@med.uni-frankfurt.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/11/PXD029794

PRIDE project URI

• PRIDE
  1. PXD029794
     1. Label: PRIDE project
     2. Name: Profiling of protein interactions of the circRNA cZNF292 in endothelial cells