PXD029780-1

PXD029780 is an original dataset announced via ProteomeXchange.

Title	A Sensitive and Controlled Data-Independent Acquisition Method for Proteomic Analysis of Cell Therapies
Description	Mass Spectrometry (MS)-based proteomic measurements are uniquely poised to impact the development of cell and gene therapies. With the adoption of rigorous instrumental performance controls (PQs), large-scale proteomics can move from a research to a manufacturing control tool. Especially suited, data-independent acquisition (DIA) approaches have distinctive qualities to extend multi-attribute method (MAM) principles to characterize the proteome of cell therapies. Here we describe the development of a DIA method for the sensitive identification and quantification of proteins on a Q-TOF instrument. Using the improved acquisition parameters, we defined some control metrics to improve the reproducibility of SWATH acquisition-based proteomic measurements. Finally, we applied the method to analyze the proteome of Jurkat cells that here serves as a model for human T-cells.
HostingRepository	PRIDE
AnnounceDate	2022-04-13
AnnouncementXML	Submission_2022-04-13_03:45:50.842.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Camille Lombard-Banek
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	iodoacetic acid derivatized residue
Instrument	TripleTOF 6600

Revision	Datetime	Status	ChangeLog Entry
0	2021-11-17 01:04:03	ID requested
⏵ 1	2022-04-13 03:45:51	announced

Dataset with its publication pending

submitter keyword: SWATH acquisition, Data-independent acquisition, Proteomics, CAR-T cells, Jurkat cells, performance control

John E. Schiel
contact affiliation	National Institute of Standards and Technology Institute for Bioscience and Biotechnology Research
contact email	john.schiel@nist.gov
lab head
Camille Lombard-Banek
contact affiliation	National Institutes of Standards and Technology/Institute for Biosciences and Bioengineering Research
contact email	clombard@umd.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/04/PXD029780

PRIDE project URI

• PRIDE
  1. PXD029780
     1. Label: PRIDE project
     2. Name: A Sensitive and Controlled Data-Independent Acquisition Method for Proteomic Analysis of Cell Therapies