PXD029766-1

PXD029766 is an original dataset announced via ProteomeXchange.

Title	Mesenchymal stem cell-derived extracellular vesicles
Description	Therapeutic benefits of mesenchymal stem/stromal cells (MSCs) are now widely believed to come from their paracrine signalling, i.e. secreted factors such as cytokines, chemokines, and extracellular vesicles (EVs). Cell-free therapy using EVs is an active and emerging field in regenerative medicine. The cellular environment of MSCs is of critical importance when directing paracrine activity. Typical 2D cultivation of stem cells on tissue culture plastic is far removed from the physiological environment of MSCs. The application of 3D cell culture allows MSCs to adapt to their cellular niche environment which, in turn, influences their paracrine signalling activity. In this study we evaluated the impact of 3D MSCs culture on EVs secretion and cargo proteome composition and functional assessment. The outcome highlights critical differences between MSC-EVs obtained from different culture microenvironments, which should be considered when scaling up MSC culture for clinical manufacturing.
HostingRepository	PRIDE
AnnounceDate	2021-11-18
AnnouncementXML	Submission_2021-11-18_06:47:01.209.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	david greening
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2021-11-16 00:48:14	ID requested
⏵ 1	2021-11-18 06:47:01	announced

Dataset with its publication pending

submitter keyword: mesenchymal stem cell, culture, extracellular vesicles, lung fibrosis

David Greening
contact affiliation	Baker Heart and Diabetes Institute
contact email	david.greening@baker.edu.au
lab head
david greening
contact affiliation	Baker Heart and Diabetes Inst.
contact email	david.greening@baker.edu.au
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD029766
     1. Label: PRIDE project
     2. Name: Mesenchymal stem cell-derived extracellular vesicles