PXD029747-1

PXD029747 is an original dataset announced via ProteomeXchange.

Title	Whole-cell abundance profiling and interaction analysis of UBQLN2
Description	To address whether expression of ALS/FTD-associated UBQLN2 mutant variants changes the cellular proteome, we performed label-free quantification-based global protein abundance profiling of patient-derived LCLs and CRISPR engineered HeLa cells. UBQLN2 mutant in both cell lines carried the mutation T487I or P497S both of which were reported to cause ALS (Deng et al., 2011; Williams et al, 2012). In addition, HeLa cells stably expressing HA-tagged MAP1B (HA-MAP1B) were subjected to HA immunoprecipitation (IP) followed by mass spectrometry to identify MAP1B interacting proteins.
HostingRepository	PRIDE
AnnounceDate	2022-06-24
AnnouncementXML	Submission_2022-06-24_02:44:54.176.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Christian Behrends
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	carbamoylated residue; monohydroxylated residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2021-11-15 06:38:18	ID requested
⏵ 1	2022-06-24 02:44:54	announced

Dataset with its publication pending

submitter keyword: UBQLN2, whole proteome, HA-IP, HeLa, LCL, ALS/FTD

Christian Behrends
contact affiliation	Munich Cluster of Systems Neurology, LMU Munich, Germany
contact email	christian.behrends@mail03.med.uni-muenchen.de
lab head
Christian Behrends
contact affiliation	SyNergy, LMU München
contact email	christian.behrends@mail03.med.uni-muenchen.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/06/PXD029747

PRIDE project URI

• PRIDE
  1. PXD029747
     1. Label: PRIDE project
     2. Name: Whole-cell abundance profiling and interaction analysis of UBQLN2