PXD029727-1

PXD029727 is an original dataset announced via ProteomeXchange.

Title	Conservation and divergence of myelin proteome profiles between humans and mice
Description	While myelin is commonly assessed in mice as a model for humans, it remained unclear to which extent their myelin protein composition is similar. We analyzed the proteome of myelin biochemically purified from human white matter by two steps of discontinuous sucrose density gradient centrifugation intermitted by osmotic shocks. We utilized a data-independent acquisition (DIA) workflow with alternating low and elevated energy (MSE) and an ion mobility-enhanced version thereof (referred to as UDMSE) to achieve both, a correct quantification of exceptionally abundant myelin proteins and a comprehensive coverage of the myelin proteome. Label-free protein quantification revealed that the relative abundance of the structural myelin proteins PLP, MBP, CNP and SEPTIN8 correlates well with that in c57Bl/6N-mice. Conversely, multiple other proteins were identified exclusively or predominantly in human or mouse myelin. Species-dependent diversity of myelin protein composition can be instructive when translating from mouse models to humans.
HostingRepository	PRIDE
AnnounceDate	2022-05-16
AnnouncementXML	Submission_2022-05-16_04:28:19.257.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Olaf Jahn
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Synapt MS

Revision	Datetime	Status	ChangeLog Entry
0	2021-11-12 07:33:28	ID requested
⏵ 1	2022-05-16 04:28:20	announced

Gargareta VI, Reuschenbach J, Siems SB, Sun T, Piepkorn L, Mangana C, Sp, ä, te E, Goebbels S, Huitinga I, M, ö, bius W, Nave KA, Jahn O, Werner HB, Conservation and divergence of myelin proteome and oligodendrocyte transcriptome profiles between humans and mice. Elife, 11():(2022) [pubmed]

submitter keyword: Oligodendrocyte, myelin proteome, central nervous system (CNS), human post mortem brain tissue, autopsy, label-free proteomics, mass spectrometry, translation

Prof. Dr. Olaf Jahn
contact affiliation	Proteomics Group Max Planck Institute of Experimental Medicine Hermann-Rein-Str. 3 37075 Goettingen, Germany Tel: +49-551-3899313 Fax: +49-551-3899323
contact email	jahn@em.mpg.de
lab head
Olaf Jahn
contact affiliation	Proteomics Group, Max-Planck-Institute of Experimental Medicine, Göttingen
contact email	jahn@em.mpg.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/05/PXD029727

PRIDE project URI

• PRIDE
  1. PXD029727
     1. Label: PRIDE project
     2. Name: Conservation and divergence of myelin proteome profiles between humans and mice