PXD029717-1

PXD029717 is an original dataset announced via ProteomeXchange.

Title	Venoms for all occasions: the functional toxin profiles of different anatomical regions in sea anemones are related to their ecological function
Description	Phylum Cnidaria is the oldest extant venomous group and is defined by the presence of nematocysts, specialised organelles responsible for venom production and delivery. While nematocysts and toxin peptides are distributed across the entire animal, nematocyst and venom profiles have been shown to differ across morphological structures in actiniarians. In this study, we explore the relationship between patterns of toxin expression and the ecological roles of discrete anatomical structures in Telmatactis stephensoni. Specifically, using a combination of proteomic and transcriptomic approaches, we examined whether there is a direct correlation between the functional similarity of regions and the similarity of their associated toxin expression profiles. We report that the regionalisation of toxin production is consistent with the partitioning of the ecological roles of venom across envenomating structures, and that three major functional regions are present in T. stephensoni— tentacles, epidermis and gastrodermis. Additionally, we find that structures which serve similar functions not only have comparable toxin profiles but also similar nematocyst types. There was no overlap in the toxins identified using proteomics and transcriptomics, however, the expression patterns of specific milked venom peptides were conserved across RNA-seq and mass spectrometry imaging datasets. Furthermore, based on our data, it appears that acontia of T. stephensoni may be transcriptionally inactive and only mature nematocyst are present in the distal portions of the threads. Overall, we find that the venom profile of different anatomical regions in sea anemones varies according to its ecological functions.
HostingRepository	PRIDE
AnnounceDate	2022-05-30
AnnouncementXML	Submission_2022-05-30_05:33:07.146.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Eivind Undheim
SpeciesList	scientific name: Telmatactis; NCBI TaxID: 1143179;
ModificationList	No PTMs are included in the dataset
Instrument	TripleTOF 5600

Revision	Datetime	Status	ChangeLog Entry
0	2021-11-12 06:11:45	ID requested
⏵ 1	2022-05-30 05:33:07	announced

Ashwood LM, Undheim EAB, Madio B, Hamilton BR, Daly M, Hurwood DA, King GF, Prentis PJ, Venoms for all occasions: The functional toxin profiles of different anatomical regions in sea anemones are related to their ecological function. Mol Ecol, 31(3):866-883(2022) [pubmed]

submitter keyword: Actiniaria

venom

toxins

transcriptomics

proteomics

spatiotemporal expression

Eivind Undheim
contact affiliation	University of Oslo, department of biosciences
contact email	e.undheim@ibv.uio.no
lab head
Eivind Undheim
contact affiliation	University of Queensland
contact email	eivindandreas@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD029717
     1. Label: PRIDE project
     2. Name: Venoms for all occasions: the functional toxin profiles of different anatomical regions in sea anemones are related to their ecological function