PXD029715-3

PXD029715 is an original dataset announced via ProteomeXchange.

Title	Targeting mitochondrial reactive oxygen species alleviates allergic airway disease in a mouse model of obese allergic asthma
Description	Obesity is associated with severe, difficult to control asthma, and increased airway oxidative stress. Mitochondrial reactive oxygen species (mROS) are an important source of oxidative stress leading us to hypothesize that targeting mROS in obese allergic asthma might be an effective treatment strategy. Using a mouse model of house dust mite (HDM) induced allergic airway disease in mice fed a low- (LFD) or high-fat diet (HFD), and the mitochondrial antioxidant MitoQuinone (MitoQ); we investigated the effects of obesity and mROS on airway inflammation, remodelling and airway hyperreactivity (AHR). HDM induces airway inflammation, remodelling and hyperreactivity in both lean and obese mice. Obese allergic mice showed increased lung tissue eotaxin levels, airway tissue eosinophilia and AHR when compared to lean allergic mice. MitoQ reduced markers of airway inflammation, remodelling and hyperreactivity in both lean and obese allergic mice, and tissue eosinophilia in obeseHDM mice. mROS regulates cell signalling by protein oxidation of multiple downstream targets: MitoQ reduced HDM-induced cysteine-sulfenylation of several proteins including those involved in the unfolded protein response (UPR). In summary, mROS mediates the development of allergic airway disease and hence MitoQ might be effective for the treatment for asthma, and specific features of obese asthma.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_05:46:26.363.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD029715
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Ying Wai Lam
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; scientific name: Bos taurus (Bovine); NCBI TaxID: 9913;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive Plus

Revision	Datetime	Status	ChangeLog Entry
0	2021-11-12 06:07:32	ID requested
1	2023-06-02 12:10:11	announced
2	2023-11-14 08:48:35	announced	2023-11-14: Updated project metadata.
⏵ 3	2024-10-22 05:46:27	announced	2024-10-22: Updated project metadata.

Chandrasekaran R, Bruno SR, Mark ZF, Walzer J, Caffry S, Gold C, Kumar A, Chamberlain N, Butzirus IM, Morris CR, Daphtary N, Aliyeva M, Lam YW, van der Vliet A, Janssen-Heininger Y, Poynter ME, Dixon AE, Anathy V, Mitoquinone mesylate attenuates pathological features of lean and obese allergic asthma in mice. Am J Physiol Lung Cell Mol Physiol, 324(2):L141-L153(2023) [pubmed]

10.1152/ajplung.00249.2022;

10.6019/PXD029715;

submitter keyword: Obesity, mitochondrial reactive oxygen species (mROS), allergic asthma, airway oxidome, unfolded protein response (UPR)., MitoQuinone, tissue eosinophilia

Vikas Anathy
contact affiliation	Associate Professor Dept. of Pathology and Laboratory Medicine HSRF 218, University of Vermont Larner College of Medicine Burlington, Vermont, 05405, USA
contact email	vikas.anathy@med.uvm.edu
lab head
Ying Wai Lam
contact affiliation	Vermont Genetics Network Proteomics Facility Department of Biology University of Vermont
contact email	ylam@uvm.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD029715
     1. Label: PRIDE project
     2. Name: Targeting mitochondrial reactive oxygen species alleviates allergic airway disease in a mouse model of obese allergic asthma