PXD029645 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | The tumor suppressor Fhit in complex with the alarmone Ap3A binds to the ribosome and impedes translation |
Description | Upon cellular stress, the concentration of the alarmone diadenosine triphosphate (Ap3A) increases and is thought to trigger downstream adaptive processes. The known Ap3A-hydrolase fragile histidine triad (Fhit) is known to form a complex with Ap3A. The molecular mechanism of Fhit-Ap3A signaling is however unknown. In this study, by synthesizing a covalent Fhit-Ap3A complex we have elucidated the Ap3A-dependent cellular interactome by mass spectrometry-based proteome profiling. Interestingly, we found a significant enrichment of proteins involved in translation. We then show that Fhit translocates from the nucleolus into the cytosol upon stress to form a Fhit-Ap3A complex which impedes translation both in vitro and in vivo in an Ap3A dependent manner resulting in reduced cell viability. Overall, our findings provide a mechanistic model by which the tumor suppressor Fhit collaborates with the alarmone Ap3A to regulate cellular proliferation. |
HostingRepository | PRIDE |
AnnounceDate | 2022-12-20 |
AnnouncementXML | Submission_2022-12-20_06:07:24.272.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | FlorianStengel |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | Q Exactive HF; Orbitrap Fusion |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2021-11-10 07:33:50 | ID requested | |
⏵ 1 | 2022-12-20 06:07:25 | announced | |
2 | 2023-11-14 08:32:55 | announced | 2023-11-14: Updated project metadata. |
Publication List
Herzog D, Jansen J, Mi, ß, un M, Diederichs K, Stengel F, Marx A, A Elucidates Its Inhibitory Action on Translation. J Am Chem Soc, 144(19):8613-8623(2022) [pubmed] |
Keyword List
submitter keyword: AP-MS, mass spectrometry, ribosome, XL-MS,Fhit, Ap3A, translation |
Contact List
AndreasMarx |
contact affiliation | Department of Chemistry University of Konstanz |
contact email | andreas.marx@uni-konstanz.de |
lab head | |
FlorianStengel |
contact affiliation | Universität Konstanz |
contact email | florian.stengel@uni-konstanz.de |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD029645
- Label: PRIDE project
- Name: The tumor suppressor Fhit in complex with the alarmone Ap3A binds to the ribosome and impedes translation