PXD029627-1

PXD029627 is an original dataset announced via ProteomeXchange.

Title	Comparative O-GlcNAc Proteomic Analysis Reveals a Role of O-GlcNAcylated SAM68 in Lung Cancer Aggressiveness
Description	O-GlcNAcylation is a reversible and dynamic post-translational protein modification catalyzed by O-GlcNAc transferase (OGT). Despite the reported association of O-GlcNAcylation with cancer metastasis, the O-GlcNAc proteome profile for cancer aggressiveness remains largely uncharac-terized. Here we report our comparative O-GlcNAc proteome profiling of 2 differentially invasive lung adenocarcinoma cell lines, which identified 158 down-regulated and 106 up-regulated can-didates in highly invasive cells. Among these differential proteins, a nuclear RNA-binding protein SAM68 (SRC associated in mitosis of 68 kDa) was further investigated. Results showed that SAM68 is O-GlcNAcylated and may interact with OGT in the nucleus. Eleven O-GlcNAcylation sites were identified, and data from mutant analysis suggested that multiple serine residues in the N-terminal region are important for O-GlcNAcylation and the function of SAM68 in modulating cancer cell migration and invasion. Analysis of clinical specimens found that high SAM68 ex-pression was associated with late cancer stages, and patients with high-OGT/high-SAM68 ex-pression in their tumors had poorer overall survival compared to those with low-OGT/low-SAM68 expression. Our study has revealed an invasiveness-associated O-GlcNAc proteome profile and connected O-GlcNAcylated SAM68 to lung cancer aggressiveness.
HostingRepository	PRIDE
AnnounceDate	2022-02-17
AnnouncementXML	Submission_2022-02-17_03:01:04.599.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD029627
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Chen-Chung Liao
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	carbamoylated residue
Instrument	LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2021-11-09 05:40:26	ID requested
⏵ 1	2022-02-17 03:01:05	announced

Lin CH, Liao CC, Wang SY, Peng CY, Yeh YC, Chen MY, Chou TY, -GlcNAcylated SAM68 in Lung Cancer Aggressiveness. Cancers (Basel), 14(1):(2022) [pubmed]

submitter keyword: Lung adenocarcinoma

O-GlcNAc transferase

O-GlcNAcylation

SAM68

Chen-Chung Liao
contact affiliation	Metabolomics-Proteomics Research Center, National Yang Ming Chiao Tung University, Taipei 11221, Taiwan
contact email	ccliao@ym.edu.tw
lab head
Chen-Chung Liao
contact affiliation	Proteomics Research Center,National Yang-Ming University
contact email	ccliao@ym.edu.tw
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD029627
     1. Label: PRIDE project
     2. Name: Comparative O-GlcNAc Proteomic Analysis Reveals a Role of O-GlcNAcylated SAM68 in Lung Cancer Aggressiveness