PXD029616-1

PXD029616 is an original dataset announced via ProteomeXchange.

Title	N-Acetylation of secreted proteins is widespread in Apicomplexa and independent of acetyl-CoA transporter AT1
Description	Acetyl-CoA critically participates in post-translational modification of proteins, central carbon and lipid metabolism in several compartments of eukaryotic cells. In mammals, the acetyl-CoA transporter 1 (AT1) facilitates the flux of cytosolic acetyl-CoA into the endoplasmic reticulum (ER) enabling the acetylation of proteins of the secretory pathway in concert with dedicated acetyltransferases including Nat8. Homologues of AT1 and Nat8 were identified in the apicomplexan parasites Toxoplasma gondii and Plasmodium berghei. However, the implication of acetyl-CoA pool influx into the ER in acetylation of ER-transiting proteins and their relevance throughout the parasites’ life cycle is unknown. Here, we report proteome-wide analyses, which revealed unprecedented widespread N-terminal acetylation marks of secreted proteins in both parasites. Deletion of the gene coding for AT1 in both parasites, resulted in global loss of fitness and in addition to retardation in erythrocytic development, malaria parasites are blocked in transmission to mosquitoes. However, in absence of AT1 proteome wide lysine and N-terminal acetylation modifications remain unaltered. This highlights a role of AT1 in parasite development uncoupled to acetylation capacity, indicative of an unusually active acetylation machinery occurring in the ER of Apicomplexa.
HostingRepository	PRIDE
AnnounceDate	2022-06-20
AnnouncementXML	Submission_2022-06-20_06:32:10.059.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Jean Baptiste BOYER
SpeciesList	scientific name: Toxoplasma gondii; NCBI TaxID: 5811; scientific name: Plasmodium berghei; NCBI TaxID: 5821;
ModificationList	acetylated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2021-11-08 09:10:43	ID requested
⏵ 1	2022-06-20 06:32:10	announced
2	2023-11-14 08:54:22	announced	2023-11-14: Updated project metadata.

10.1242/JCS.259811;

submitter keyword: Toxoplasma gondii, Plasmodium berghei, Acetyl-CoA transporter, Acetylome, Secretory pathway, Endoplasmic Reticulum

Carmela GIGLIONE
contact affiliation	Protein Maturation, Cell fate and Therapeutics Institute for Integrative Biology of the Cell (I2BC) CNRS UMR9198, Bt. 21, 1 Avenue de la Terrasse F-91198 Gif-sur-Yvette cedex, France
contact email	carmela.giglione@i2bc.paris-saclay.fr
lab head
Jean Baptiste BOYER
contact affiliation	Institut de Biologie Intégrative de la Cellule - CNRS
contact email	jean-baptiste.boyer@i2bc.paris-saclay.fr
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/06/PXD029616

PRIDE project URI

• PRIDE
  1. PXD029616
     1. Label: PRIDE project
     2. Name: N-Acetylation of secreted proteins is widespread in Apicomplexa and independent of acetyl-CoA transporter AT1