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PXD029596-1

PXD029596 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleThymine DNA glycosylase (TDG) regulates cell cycle–driven p53 transcriptional control in pluripotent cells
DescriptionCell cycle progression is linked to transcriptome dynamics and variations in the response of pluripotent cells to differentiation cues, through mostly unknown determinants. Here, we characterized the cell cycle–associated transcriptome and proteome of mouse embryonic stem cells (mESCs) in naïve ground state. We found that the thymine DNA glycosylase (TDG) is a cell cycle–regulated co-factor of the tumour suppressor p53. Further, TDG and p53 co-bind ESC-specific cis-regulatory elements and thereby control transcription of p53-dependent genes during self-renewal. We determined that the dynamic expression of TDG is required to promote the cell cycle–associated transcriptional heterogeneity. Moreover, we demonstrated that transient depletion of TDG influences cell fate decisions during the early differentiation of mESCs. Our findings reveal an unanticipated role of TDG in promoting molecular heterogeneity during the cell cycle, and highlight the central role of protein dynamics for the temporal control of cell fate during development.
HostingRepositoryPRIDE
AnnounceDate2023-07-31
AnnouncementXMLSubmission_2023-07-31_03:21:48.086.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterEvaBorràs
SpeciesList scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationListmonohydroxylated residue; iodoacetamide derivatized residue
InstrumentOrbitrap Fusion Lumos; LTQ Orbitrap Velos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02021-11-05 08:41:39ID requested
12023-07-31 03:21:48announced
22023-11-14 06:49:27announced2023-11-14: Updated project metadata.
Publication List
Keyword List
submitter keyword: cell cycle, embryonic stem cells, mass spectrometry
Contact List
EduardSabido
contact affiliationCRG, UPF
contact emaileduard.sabido@crg.eu
lab head
EvaBorràs
contact affiliationUPF
contact emaileva.borras@upf.edu
dataset submitter
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Dataset FTP location
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PRIDE project URI
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