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PXD029573-1

PXD029573 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleRole of Nse1 subunit of SMC5/6 complex as a ubiquitin ligase
DescriptionStructural Maintenance of Chromosomes (SMC) complexes - cohesin, condensin and SMC5/6 - are important for many aspects of the chromosomal organization. These complexes are composed of Smc and non-Smc element (Nse) subunits. The Nse1 subunit of SMC5/6 contains a variant RING domain, characteristic of ubiquitin ligases. Human NSE1 has been shown to possess ubiquitin ligase activity in vitro; however, other studies were unable to show such activity. Here, we confirm Nse1 ubiquitin-ligase activity in vitro using purified Schizosaccharomyces pombe proteins. We demonstrate that the Nse1 ligase activity is stimulated by Nse3 and Nse4, it specifically utilizes Ubc13/Mms2 E2 enzyme and Nse1 interacts directly with ubiquitin. We identify Nse1 mutation (R188E) that specifically disrupts its E3 activity, and show that the Nse1-dependent ubiquitination is particularly important under replication stress. Moreover, we identify Nse4 (lysine K181) as the first known SMC5/6-associated Nse1 substrate. Interestingly, abolition of Nse4 modification at K181 leads to suppression of DNA-damage sensitivity of other SMC5/6 mutants. Altogether, this study brings new evidence for Nse1 ubiquitin ligase activity, significantly advancing our under-standing of this enigmatic SMC5/6 function.
HostingRepositoryPRIDE
AnnounceDate2022-02-19
AnnouncementXMLSubmission_2022-02-19_11:22:22.247.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterDavid Potesil
SpeciesList scientific name: Schizosaccharomyces pombe 927; NCBI TaxID: 1264690; scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListubiquitination signature dipeptidyl lysine
InstrumentLTQ Orbitrap Elite
Dataset History
RevisionDatetimeStatusChangeLog Entry
02021-11-04 10:17:38ID requested
12022-02-19 11:22:22announced
22023-11-14 08:54:12announced2023-11-14: Updated project metadata.
Publication List
10.3390/CELLS11010165;
Keyword List
submitter keyword: SMC5/6
Nse1, ubiquitin ligase, ubiquitination, liquid chromatography, mass spectrometry
Contact List
Zbynek Zdrahal
contact affiliationCEITEC—Central European Institute of Technology, Masaryk University, Brno, Czech Republic
contact emailzdrahal@sci.muni.cz
lab head
David Potesil
contact affiliationProteomics Core Facility and Research Group Proteomics CEITEC-MU Masaryk University Brno, Czech Republic
contact emaildavid.potesil@ceitec.muni.cz
dataset submitter
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Dataset FTP location
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