PXD029556-1
PXD029556 is an original dataset announced via ProteomeXchange.
Dataset Summary
Title | Efficient TurboID-based proximate labeling method for identifying terminal sialic acid glycosylation in living cells |
Description | TurboID, a mutant biotin ligase, based proximity labeling method is a new efficient technique for recognizing protein-protein interactions and has been successfully applied to living cells. Sialic acids are typically the terminal monosaccharides attached to many glycoproteins and play many important roles in many biological processes. However, the lack of enrichment methods for terminal sialic acid glycosylation in vivo hinders the identification and analysis of this glycosylation. Here, we introduced this technique to identify terminal sialic acid glycosylation in living cells. SpCBM, the carbohydrate-binding domain of sialidase from Streptococcus pneumoniae, was fused with TurboID and overexpressed in HeLa cells. After streptavidin-based purification and detection by mass spectrometry (MS), we identified 41 terminal sialic acid N-glycosylated sites and 3065 putative terminal sialic acid glycosylated proteins which many of them were located in cytoplasm and nucleus. |
HostingRepository | iProX |
AnnounceDate | 2021-11-04 |
AnnouncementXML | Submission_2023-12-21_20:51:04.600.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Wu Liu |
SpeciesList | scientific name: Homo sapiens; NCBI TaxID: 9606; |
ModificationList | No PTMs are included in the dataset |
Instrument | Orbitrap Fusion |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
---|---|---|---|
0 | 2021-11-04 00:07:02 | ID requested | |
⏵ 1 | 2023-12-21 20:51:05 | announced |
Publication List
Liu W, Long Y, Bao Y, Li Y, Deng M, Yang X, Zhu H, Su Y, Efficient TurboID-based proximity labelling method for identifying terminal sialic acid glycosylation in living cells. Acta Biochim Biophys Sin (Shanghai), 54(12):1841-1853(2022) [pubmed] |
Keyword List
submitter keyword: TurboID, proximity lableing, glycosylation, sialic acid, SpCBM |
Contact List
Yanting Su | |
---|---|
contact affiliation | Research Center of Basic Medical Sciences, School of Basic Medical Sciences, Hubei University of Science and Technology |
contact email | 2014202040134@whu.edu.cn |
lab head | |
Wu Liu | |
contact affiliation | Hubei Key Laboratory of Diabetes and Angiopathy, Hubei University of Science and Technology |
contact email | lw463684057@163.com |
dataset submitter |
Full Dataset Link List
iProX dataset URI |