PXD029552-1

PXD029552 is an original dataset announced via ProteomeXchange.

Title	C. difficile strain 630 flagellin C modifications
Description	In proteomics, the study of post-translational modifications often relies on some type of enrichment strategy. Here, we show that an approach that is commonly used in phosphoproteomics (Immobilised Metal Affinity Chromatography (Fe3+-IMAC)) also enriches for peptides with a unique post-translational modification, known as type A, in the human pathogen Clostridioides difficile. The type A modification consists of a monosaccharide (GlcNAc) that is linked to an N-methylated threonine through a phosphodiester bond. This structure has previously been described on flagellin C of several C. difficile strains and is important for bacterial motility. Using LC-MS/MS analyses of IMAC-captured tryptic peptides, we not only observed Type A modified C. difficile flagellin peptides but also a variety of truncated/modified Type A structures on these peptides. Using an elaborate set of mass spectrometry analyses, we demonstrate that one of these modifications consists of a Type A structure containing a phosphonate (2-aminoethylphosphonate, 2-AEP), a modification that is rarely observed and has hitherto not been described in C. difficile.
HostingRepository	PRIDE
AnnounceDate	2022-04-04
AnnouncementXML	Submission_2022-04-03_18:23:47.246.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Yassene Mohammed
SpeciesList	scientific name: Clostridioides difficile 630; NCBI TaxID: 272563;
ModificationList	monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2021-11-03 06:33:02	ID requested
⏵ 1	2022-04-03 18:23:48	announced

Hensbergen PJ, de Ru AH, Friggen AH, Corver J, Smits WK, van Veelen PA, New insights into the type A glycan modification of Clostridioides difficile flagellar protein flagellin C by phosphoproteomics analysis. J Biol Chem, 298(3):101622(2022) [pubmed]

submitter keyword: C. difficile, phosphoproteomics, flagellin

Paul J. Hensbergen
contact affiliation	Center for Proteomics and Metabolomics Leiden University Medical Center Albinusdreef 2 2333 ZA Leiden The Netherlands
contact email	P.J.Hensbergen@lumc.nl
lab head
Yassene Mohammed
contact affiliation	LUMC/UVIC
contact email	y.mohammed@lumc.nl
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/04/PXD029552

PRIDE project URI

• PRIDE
  1. PXD029552
     1. Label: PRIDE project
     2. Name: C. difficile strain 630 flagellin C modifications