PXD029511-1

PXD029511 is an original dataset announced via ProteomeXchange.

Title	Comparative proteomic analysis reveals the different hepatotoxic mechanisms of human hepatocytes exposed to silver nanoparticles
Description	Silver nanoparticles (AgNPs), which have been used extensively in consuming products and eventually released into the natural environment, have aroused concerns recently because of their potentially harmful effects on human beings following various routes of exposure. As the liver is one of the largest accumulation and deposition sites of circulatory AgNPs, it is important to evaluate the hepatotoxicity induced by AgNPs. However, the acting mechanisms of AgNPs-induced hepatotoxicity are still elusive to a great extent. Herein, we investigated the hepatotoxic effects of AgNPs using a comparative proteomics approach. First, we evaluated the cytotoxicity of different-sized AgNPs and found that the cancerous liver cells were generally more sensitive than the normal liver cells. Next, proteomics results suggested that HepG2 and L02 cells showed distinct adaptive responses upon AgNPs exposure. HepG2 cells respond to stresses by adapting energy metabolism, upregulating metallothionein expression and increasing the expression of antioxidants, while L02 cells protect themselves by increasing DNA repair and macro-autophagy. Besides, mitochondrial ROS has been identified as one of the causes of AgNPs-induced hepatotoxicity. Collectively, our results revealed that hepatic cancer cells and normal cells cope with AgNPs in notably different pathways, providing new insights into mechanisms underlying AgNPs-induced hepatotoxicity.
HostingRepository	PRIDE
AnnounceDate	2022-12-21
AnnouncementXML	Submission_2022-12-21_09:45:24.609.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Tin YanWong
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	timsTOF Pro

Revision	Datetime	Status	ChangeLog Entry
0	2021-11-02 06:51:00	ID requested
⏵ 1	2022-12-21 09:45:24	announced
2	2023-11-14 08:54:28	announced	2023-11-14: Updated project metadata.

submitter keyword: Human hepatocytes, silver nanoparticles

HenryLam
contact affiliation	HKUST
contact email	kehlam@ust.hk
lab head
Tin YanWong
contact affiliation	The Hong Kong University of Science and Technology
contact email	tywongam@connect.ust.hk
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD029511
     1. Label: PRIDE project
     2. Name: Comparative proteomic analysis reveals the different hepatotoxic mechanisms of human hepatocytes exposed to silver nanoparticles