PXD029492-1

PXD029492 is an original dataset announced via ProteomeXchange.

Title	Alphavirus infection triggers selective cytoplasmic translocation of nuclear RBPs with moonlighting antiviral roles
Description	RNA is a central molecule for RNA viruses, acting as mRNA and genome. However, the interactions that viral (v)RNA establishes with the host cell is only starting to be elucidated. Here, we determine with unprecedented depth the composition of the ribonucleoproteins (RNPs) of the prototypical arthropod-borne Sindbis virus (SINV) using viral RNA interactome capture. We show that SINV RNAs engage with hundreds of cellular proteins and pathways, including a group of nuclear RNA-binding proteins (RBPs) with unknown roles in infection. Combining subcellular fractionation and proteomics with several orthogonal approaches, we demonstrate that these nuclear RBPs are selectively redistributed to the cytoplasm after infection, where they associate with the viral replication organelles. These nuclear RBPs potently supress viral gene expression, with activities spanning viral species and families. Our study provides a comprehensive and systematic analysis of SINV RNP composition, revealing a network of nuclear RBPs with moonlighting antiviral function.
HostingRepository	PRIDE
AnnounceDate	2024-12-19
AnnouncementXML	Submission_2024-12-19_14:29:57.082.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Wael Kamel
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	acetylated residue; monohydroxylated residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2021-11-02 03:54:15	ID requested
⏵ 1	2024-12-19 14:29:57	announced

Kamel W, Ruscica V, Embarc-Buh A, de Laurent ZR, Garcia-Moreno M, Demyanenko Y, Orton RJ, Noerenberg M, Madhusudhan M, Iselin L, J, ä, rvelin AI, Hannan M, Kitano E, Moore S, Merits A, Davis I, Mohammed S, Castello A, Alphavirus infection triggers selective cytoplasmic translocation of nuclear RBPs with moonlighting antiviral roles. Mol Cell, 84(24):4896-4911.e7(2024) [pubmed]

10.1016/j.molcel.2024.11.015;

submitter keyword: RNA-binding proteins, vRIC

Alfredo Castello
contact affiliation	Glasgow University
contact email	alfredo.castello@glasgow.ac.uk
lab head
Wael Kamel
contact affiliation	Glasgow university
contact email	wael.s.kamel@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/12/PXD029492

PRIDE project URI

• PRIDE
  1. PXD029492
     1. Label: PRIDE project
     2. Name: Alphavirus infection triggers selective cytoplasmic translocation of nuclear RBPs with moonlighting antiviral roles