PXD029443-1

PXD029443 is an original dataset announced via ProteomeXchange.

Title	Progressive axonopathy when oligodendrocytes lack the myelin protein CMTM5
Description	Chemokine-like factor-like MARVEL-transmembrane domain containing protein 5 (CMTM5) is a tetraspan-transmembrane protein highly enriched in oligodendrocytes and central nervous system (CNS) myelin. We analyzed the proteome of myelin biochemically purified from mouse brains upon genetic disruption of the Cmtm5-gene specifically in myelinating cells. An ion mobility-enhanced version of data-independent acquisition (DIA) workflow with alternating low and elevated energy (referred to as UDMSE) was used to compare protein abundance in Cmtm5 cKO and CTRL samples by label-free quantification. Specifically, dynamic range enhancement (DRE)-UDMSE was applied as a dedicated data acquisition mode recently introduced for routine differential myelin proteome profiling. We found that Cmtm5 cKO mice displayed a largely similar myelin proteome composition as control mice and, together with other data such as electron micrographs, concluded that CMTM5 is not essential for myelin biogenesis and composition. However, oligodendroglial Cmtm5-deficiency causes an early-onset progressive axonopathy, presumably following a Wallerian-like pathomechanism. These results indicate that CMTM5 is involved in the function of oligodendrocytes to maintain axonal integrity rather than myelin biogenesis.
HostingRepository	PRIDE
AnnounceDate	2022-05-25
AnnouncementXML	Submission_2022-05-25_07:08:47.208.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Olaf Jahn
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	No PTMs are included in the dataset
Instrument	Synapt MS

Revision	Datetime	Status	ChangeLog Entry
0	2021-10-29 08:58:48	ID requested
⏵ 1	2022-05-25 07:08:48	announced

Buscham TJ, Eichel-Vogel MA, Steyer AM, Jahn O, Strenzke N, Dardawal R, Memhave TR, Siems SB, M, ü, ller C, Meschkat M, Sun T, Ruhwedel T, M, ö, bius W, Kr, ä, mer-Albers EM, Boretius S, Nave KA, Werner HB, Progressive axonopathy when oligodendrocytes lack the myelin protein CMTM5. Elife, 11():(2022) [pubmed]

submitter keyword: Oligodendrocyte, myelin proteome, chemokine-like factor-like MARVEL-transmembrane domain containing protein (CMTM) family, central nervous system (CNS), neurodegeneration, Wallerian degeneration, label-free proteomics, mass spectrometry

Prof. Dr. Olaf Jahn
contact affiliation	Proteomics Group Max Planck Institute of Experimental Medicine Hermann-Rein-Str. 3 37075 Goettingen, Germany Tel: +49-551-3899313 Fax: +49-551-3899323
contact email	jahn@em.mpg.de
lab head
Olaf Jahn
contact affiliation	Proteomics Group, Max-Planck-Institute of Experimental Medicine, Göttingen
contact email	jahn@em.mpg.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/04/PXD029443

PRIDE project URI

• PRIDE
  1. PXD029443
     1. Label: PRIDE project
     2. Name: Progressive axonopathy when oligodendrocytes lack the myelin protein CMTM5