PXD029411-1
PXD029411 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Label free proteomic and complementary metabolomic analysis of leaves of the resurrection plant Xerophyta schlechteri during dehydration |
| Description | Vegetative desiccation tolerance, the ability to survive loss of ~ 95% relative water content (RWC) is rare in angiosperms, these being commonly called resurrection plants. It is a complex multigenic and multi-factorial trait, its understanding requiring a comprehensive systems biology approach. The aim of the current study was to conduct a label-free proteomic analysis of leaves of the res-urrection plant Xerophyta schlechteri in response to desiccation. A targeted metabolomics approach validated and correlated to the proteomics, contributing the missing link in studies on this species. Three physiological stages were identified. An early response to drying, during which the leaf tissues decline from full turgor to a relative water content (RWC) of ~ 80 - 70%, a mid-response in which RWC declines to 40% and a late response where tissues decline to 10% RWC. We identified 517 distinct proteins that were differentially expressed, of which 253 proteins were upregulated and 264 were downregulated in response to the three drying stages. Metabolomics analyses, which included monitoring levels of a selection of phytohormones, amino acids, sugars, sugar alcohols, fatty acids and organic acids in response to dehydration, correlated with some of the proteomic differences, giving insight into the biological processes apparently involved in desiccation tol-erance of this species. |
| HostingRepository | MassIVE |
| AnnounceDate | 2021-10-30 |
| AnnouncementXML | Submission_2021-10-30_01:08:03.503.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Non peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Supported dataset by repository |
| PrimarySubmitter | David L. Tabb |
| SpeciesList | scientific name: Xerophyta schlechteri; NCBI TaxID: 1289414; |
| ModificationList | Oxidation; L-cysteine methyl disulfide |
| Instrument | Q Exactive |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2021-10-28 19:32:11 | ID requested | |
| ⏵ 1 | 2021-10-30 01:08:03 | announced |
Publication List
| no publication |
Keyword List
| submitter keyword: resurrection plant, desiccation |
Contact List
| Mohammed Suhail Rafudeen | |
|---|---|
| contact affiliation | University of Cape Town |
| contact email | suhail.rafudeen@uct.ac.za |
| lab head | |
| David L. Tabb | |
| contact affiliation | Stellenbosch University |
| contact email | dtabb@sun.ac.za |
| dataset submitter | |
Full Dataset Link List
| MassIVE dataset URI |
| Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/MSV000088279/ |
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