PXD029379 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Label-free quantification (LFQ) analysis on skeletal muscle stem cells and reprogrammed skeletal muscle stem and progenitor cells |
Description | Direct lineage reprogramming provides a unique system to study cell fate transitions and unearth molecular mechanisms that safeguard cellular identity. We previously reported on direct conversion of mouse fibroblasts into induced myogenic progenitor cells (iMPCs) by transient MyoD overexpression in concert with small molecules treatment. Here we employed integrative multi-omic approaches to delineate the molecular landscape of fibroblast reprogramming into iMPCs in comparison to transdifferentiation into myogenic cells solely by MyoD overexpression. Utilizing bulk RNA-sequencing and mass spectrometry, we uncovered molecular regulators and pathways that endow a myogenic stem cell identity on fibroblasts only in the presence of small molecule treatment. In addition, we demonstrate that Pax7+ cells in iMPCs share molecular attributes with myoblasts, however in addition express unique genes, proteins and pathways that are indicative of a more activated satellite cell-like state in vitro. Collectively, this study charts a molecular blueprint for reprogramming fibroblasts into muscle stem and progenitor cells and further establishes the fidelity of stable iMPC cultures in capturing skeletal muscle regeneration in vitro for disease modeling and basic research applications. |
HostingRepository | PRIDE |
AnnounceDate | 2022-05-19 |
AnnouncementXML | Submission_2022-05-19_13:39:22.343.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Adhideb Ghosh |
SpeciesList | scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; |
ModificationList | No PTMs are included in the dataset |
Instrument | Orbitrap Fusion Lumos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2021-10-27 08:13:39 | ID requested | |
⏵ 1 | 2022-05-19 13:39:23 | announced | |
Publication List
Kim I, Ghosh A, Bundschuh N, Hinte L, Petrosyan E, von Meyenn F, Bar-Nur O, Integrative molecular roadmap for direct conversion of fibroblasts into myocytes and myogenic progenitor cells. Sci Adv, 8(14):eabj4928(2022) [pubmed] |
Keyword List
submitter keyword: Reprogramming, Skeletal muscle, Satellite cells, LFQ |
Contact List
Prof. Dr. Ori Bar-Nur |
contact affiliation | Laboratory of Regenerative and Movement Biology, Dept. of Health Sciences and Technology ETH Zurich, Schorenstrasse 16, 8603 Schwerzenbach, Switzerland |
contact email | ori.bar-nur@hest.ethz.ch |
lab head | |
Adhideb Ghosh |
contact affiliation | ETH Zurich |
contact email | adhideb.ghosh@fgcz.ethz.ch |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD029379
- Label: PRIDE project
- Name: Label-free quantification (LFQ) analysis on skeletal muscle stem cells and reprogrammed skeletal muscle stem and progenitor cells