PXD029315-1

PXD029315 is an original dataset announced via ProteomeXchange.

Title	Proteomic response to cytotoxicity of methylglyoxall
Description	Tumor glycolysis is a target for cancer chemotherapy. Methylglyoxal (MG) is a reactive metabolite formed mainly as a by-product in anaerobic glycolysis, metabolized by glyoxalase 1 (Glo1) of the glyoxalase system. MG is increased to cytotoxic levels by clinical anticancer drugs, contributing to their mechanism of action. We investigated the role of the proteomic response to MG in mediating cytotoxicity. The proteomic response to MG-induced cytotoxicity was explored by high mass resolution proteomics of cytoplasmic and other subcellular protein extracts. MG induced decrease of mitochondrial and spliceosomal proteins. Spliceosomal proteins were targets of MG modification. We conclude that MG-mediated cytotoxicity targets the spliceosome.
HostingRepository	PRIDE
AnnounceDate	2021-11-02
AnnouncementXML	Submission_2021-11-02_07:03:17.084.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Paul Thornalley
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	methylglyoxal arginine adduct (+54 amu); monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2021-10-25 00:22:07	ID requested
⏵ 1	2021-11-02 07:03:17	announced

Dataset with its publication pending

submitter keyword: methylglyoxal, cytotoxicity, cancer chemotherapy

Paul J Thornalley
contact affiliation	Hamad bin Kahlifa University, Doha Qatar
contact email	pthornalley@hbku.edu.qa
lab head
Paul Thornalley
contact affiliation	Qatar Biomedical Research Institute (QBRI), Hamad Bin Khalifa University
contact email	pthornalley@hbku.edu.qa
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/11/PXD029315

PRIDE project URI

• PRIDE
  1. PXD029315
     1. Label: PRIDE project
     2. Name: Proteomic response to cytotoxicity of methylglyoxall