PXD029312-2

PXD029312 is an original dataset announced via ProteomeXchange.

Title	Quantitative Proteomics Reveals the Dynamic Pathophysiology across Different Stages in a Rat Model of Severe Traumatic Brain Injury
Description	Background: Severe Traumatic Brain Injury (TBI) has become a growing health problem, causing a vast society burden worldwide. However, characteristic mechanisms between acute and subacute stages have not been systemically revealed. Methods: Sixty rats were randomly divided into sham surgery and model groups. Severe TBI model was induced by controlled cortical impact (CCI). Neurological deficits were evaluated by modified neurological severity score (mNSS). H&E staining and immunofluorescence were performed to assess the injured brain tissues. The protein expressions of hippocampus were analyzed by the tandem mass tag-based (TMT) quantitative proteomics on the third and fourteenth days. The shared and stage-specific differentially expressed proteins (DEPs) were screened, and then analyzed by the gene ontology (GO), Kyoto encyclopedia of genes and genomes (KEGG) and protein-protein interaction (PPI). Eventually, target proteins were further verified by Western blotting (WB). Results: The neurological deficits always exist from the acute stage to the subacute stage of severe TBI and showed a downward trend. The brain parenchyma of TBI models was dramatically impaired. A total of 375 and 139 DEPs were identified at day 3 and 14, respectively. Sixty-three DEPs were co-existed between the two stages. Both stages were mainly related to the complement and coagulation cascades pathway. Three pathways associated with gastric acid secretion, insulin secretion and thyroid hormone synthesis were only enriched in the acute phase. Comparatively, amyotrophic lateral sclerosis (ALS) was significantly enriched in the subacute stage. WB confirmed the reliability of the TMT quantitative proteomics results. Conclusion: Our findings highlight the same and different pathological processes in the acute and subacute phases of severe TBI and provide potential protein biomarkers.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:48:34.127.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Weikang Luo
SpeciesList	scientific name: Rattus norvegicus (Rat); NCBI TaxID: 10116;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2021-10-25 00:02:51	ID requested
1	2023-06-07 07:47:35	announced
⏵ 2	2023-11-14 08:48:36	announced	2023-11-14: Updated project metadata.
3	2024-10-22 05:47:09	announced	2024-10-22: Updated project metadata.

Luo W, Yang Z, Zhang W, Zhou D, Guo X, Wang S, He F, Wang Y, Quantitative Proteomics Reveals the Dynamic Pathophysiology Across Different Stages in a Rat Model of Severe Traumatic Brain Injury. Front Mol Neurosci, 14():785938(2021) [pubmed]

submitter keyword: acute phase, severe traumatic brain injury, subacute phase, characteristic mechanisms,quantitative proteomic, tandem mass tag-based

Yang Wang
contact affiliation	Xiangya Hospital Central South University
contact email	wangyang_xy87@csu.edu.cn
lab head
Weikang Luo
contact affiliation	Xiangya Hospital Central South University
contact email	wk1996@csu.edu.cn
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD029312
     1. Label: PRIDE project
     2. Name: Quantitative Proteomics Reveals the Dynamic Pathophysiology across Different Stages in a Rat Model of Severe Traumatic Brain Injury