PXD029277-1

PXD029277 is an original dataset announced via ProteomeXchange.

Title	SS18/BAFs regulate chromatin accessibility dynamics during pluripotent-somatic transition
Description	The exit from pluripotency or pluripotent-somatic transition (PST) landmarks an event of mammalian development, and is also a representative cell-fate transition model, but remains largely unresolved. Recently, we reported construction of robust JUN-induced PST completed in one cell cycle and whose dominant regulator SS18/BAFs (Brg1/Brahma-associated factors). However, the transition process in the chromatin architecture and the roles played by BAF are still unknown. Here we report the dynamic changes of chromatin accessibility during JUN-induced PST. Meanwhile, SS18/BAFs mediates PST process by relocating from pluripotent loci to AP-1 associated ones and once compromised, JUN fails to open chromatin and PST will be delayed. Furthermore, we show that the relocation of SS18/BAF partially relays on histone modification H3K27ac, instead of JUN-centric protein-protein interaction. These results reveal the orchestration of master transcription factor, epigenetic machine, and histone modification in the cell fate transition.
HostingRepository	iProX
AnnounceDate	2021-10-22
AnnouncementXML	Submission_2021-10-21_21:51:57.776.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Ziwei Zhai
SpeciesList	scientific name: Mus musculus; NCBI TaxID: 10090;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2021-10-21 21:51:39	ID requested
⏵ 1	2021-10-21 21:51:58	announced
2	2022-10-13 23:44:27	announced	2022-10-14: Update publication information.

Dataset with its publication pending

submitter keyword: JUN, SS18/BAFs complex, pluripotent-somatic transition, mouse embryonic stem cells, chromatin accessibility, cell fate determination

Jing Liu
contact affiliation	Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences
contact email	liu_jing@gibh.ac.cn
lab head
Ziwei Zhai
contact affiliation	Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences
contact email	zhai_ziwei@gibh.ac.cn
dataset submitter

iProX dataset URI