PXD029227-1

PXD029227 is an original dataset announced via ProteomeXchange.

Title	Characterising the RNA-binding protein atlas of the mammalian brain uncovers RBM5 misregulation in mouse models of Huntington’s disease
Description	RNA-binding proteins (RBPs) are key players regulating RNA processing and are associated with disorders ranging from cancer to neurodegeneration. Here, we present a proteomics workflow for large-scale identification of RBPs and their RNA-binding regions in the mammalian brain identifying 526 RBPs including 86 previously undescribed. Analysing brain tissue from the Huntington’s disease (HD) R6/2 mouse model uncovered differential RNA-binding of the alternative splicing regulator RBM5. Combining several omics workflows, we show that RBM5 binds differentially to transcripts enriched in pathways of neurodegeneration in R6/2 brain tissue. We further find these transcripts to undergo changes in splicing and demonstrate that RBM5 directly regulates these changes in human neurons derived from embryonic stem cells. Finally, we reveal that RBM5 interacts differently with several known huntingtin interactors and components of huntingtin aggregates. Collectively, we demonstrate the applicability of our method for capturing RNA interactor dynamics in the contexts of tissue and disease.
HostingRepository	PRIDE
AnnounceDate	2023-06-22
AnnouncementXML	Submission_2023-06-22_02:14:21.608.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	MeeliMullari
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF; Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2021-10-20 03:05:02	ID requested
⏵ 1	2023-06-22 02:14:21	announced
2	2024-10-22 05:49:38	announced	2024-10-22: Updated project metadata.

Dataset with its publication pending

submitter keyword: RBM5, Mass Spectrometry,Brain-pCLAP, RNA-protein interactions, RNA-binding proteins, Huntington’s Disease

Michael LundNielsen
contact affiliation	NNF CPR, Copenhagen Univeristy, Denmark
contact email	michael.lund.nielsen@cpr.ku.dk
lab head
MeeliMullari
contact affiliation	Copenhagen University
contact email	meeli.mullari@cpr.ku.dk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/06/PXD029227

PRIDE project URI

• PRIDE
  1. PXD029227
     1. Label: PRIDE project
     2. Name: Characterising the RNA-binding protein atlas of the mammalian brain uncovers RBM5 misregulation in mouse models of Huntington’s disease