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PXD029213-1

PXD029213 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleThe effects of mild intermittent hypoxia exposure on the abdominal subcutaneous adipose tissue proteome in overweight and obese men: A first-in-human randomized, single-blind, cross-over study
DescriptionAdipose tissue (AT) oxygen tension (pO2) has been implicated in AT dysfunction and metabolic perturbations in both rodents and humans. Compelling evidence suggests that hypoxia exposure alters metabolism, at least partly through effects on AT. However, it remains to be elucidated whether mild intermittent hypoxia (MIH) exposure impacts the AT proteome. We performed a randomized, single-blind, cross-over study to investigate the effects of seven consecutive days of MIH (FiO2 15%, 3x2h/d) compared to normoxia (FiO2 21%) exposure, on the AT proteome in overweight/obese men. AT insulin sensitivity was determined by a two-step hyperinsulinemic-euglycemic clamp, and abdominal subcutaneous AT biopsies were collected (n=11) under normoxic, fasting conditions following both exposure regimens. AT proteins were isolated and quantified using liquid chromatography-mass spectrometry. After correction for blood contamination, 1022 AT protein IDs were identified, of which 123 were differentially expressed following MIH (p < 0.05). These proteins were involved in redox systems, cell-adhesion, actin cytoskeleton organization, extracellular matrix composition and energy metabolism. We demonstrate for the first time that MIH exposure impacts the AT proteome. Moreover, differential AT TMOD3 expression is related to changes in AT insulin sensitivity, thereby linking MIH-induced effects on the AT proteome to metabolic changes in overweight/obese humans.
HostingRepositoryPRIDE
AnnounceDate2022-02-16
AnnouncementXMLSubmission_2022-02-16_08:14:25.601.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterfreek bouwman
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListacetylated residue; iodoacetamide derivatized residue
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02021-10-19 01:46:07ID requested
12022-02-16 08:14:26announced
Publication List
Van Meijel RLJ, Wang P, Bouwman F, Blaak EE, Mariman ECM, Goossens GH, The Effects of Mild Intermittent Hypoxia Exposure on the Abdominal Subcutaneous Adipose Tissue Proteome in Overweight and Obese Men: A First-in-Human Randomized, Single-Blind, and Cross-Over Study. Front Physiol, 12():791588(2021) [pubmed]
Keyword List
submitter keyword: Human hypoxia adipose tissue
Contact List
Freek Bouwman
contact affiliationdept. Human Biology Maastricht University The Netherlands
contact emailfreek.bouwman@maastrichtuniversity.nl
lab head
freek bouwman
contact affiliationHuman Biology
contact emailfreek.bouwman@maastrichtuniversity.nl
dataset submitter
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Dataset FTP location
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