PXD029205-1

PXD029205 is an original dataset announced via ProteomeXchange.

Title	Quantitative proteomic dataset of the moss Physcomitrium patens SMG1 KO mutant line
Description	Here, we provide an isobaric tag for relative and absolute quantitation (iTRAQ)-based quantitative proteomic dataset of the moss Physcomitrium patens smg1 knockout line. The kinase SMG1 is one of the key components of the NMD system in many organisms, including plants. Several lines with a deleted SMG1 in a land Physcomitrium patens subsp. patens (“Gransden 2004”, Frieburg) were produced by James P. B. Lloyd [doi.org/10.1111/tpj.12329]. One of these lines, SMG1 KO mutant line 2, was used for this study. Protonemata of WT and mutant lines were grown in200 ml liquid BCD medium supplemented with 5 mM ammonium tartrate (BCDAT) during a 16-h photoperiod at 250C for 8 days [https://doi.org/10.1101/pdb.prot5136]. All experiments were performed in three biological replicates.
HostingRepository	PRIDE
AnnounceDate	2021-11-02
AnnouncementXML	Submission_2021-11-02_06:47:45.334.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Igor Fesenko
SpeciesList	scientific name: Physcomitrium patens; NCBI TaxID: 3218;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2021-10-18 05:20:06	ID requested
⏵ 1	2021-11-02 06:47:45	announced

Dataset with its publication pending

submitter keyword: Physcomitrium patens, iTRAQ, NMD

Igor Fesenko
contact affiliation	IBCH RAS
contact email	fesigor@gmail.com
lab head
Igor Fesenko
contact affiliation	IBCH RAS
contact email	fesigor@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD029205
     1. Label: PRIDE project
     2. Name: Quantitative proteomic dataset of the moss Physcomitrium patens SMG1 KO mutant line