PXD029191-1

PXD029191 is an original dataset announced via ProteomeXchange.

Title	OpenCell: proteome-scale endogenous tagging enables the cartography of human cellular organization
Description	Elucidating the wiring diagram of the human cell is one of the central goals of the post-genomic era. Here, we integrate genome engineering, confocal live-cell imaging, mass spectrometry and data science to systematically map the localization and interactions of human proteins. Our approach provides a data-driven description of the molecular and spatial networks that organize the proteome. Unsupervised clustering of these networks delineates functional communities that facilitate biological discovery, and uncovers that RNA-binding proteins form a specific sub-group defined by unique interaction and localization properties. Furthermore, we discover that localization patterns are remarkably predictive of protein function and often contain enough information to identify molecular interactions. Paired with a fully interactive website (opencell.czbiohub.org), we provide a resource for the quantitative cartography of human cellular organization.
HostingRepository	PRIDE
AnnounceDate	2022-03-04
AnnouncementXML	Submission_2022-03-04_10:46:04.547.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD029191
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Frank McCarthy
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2021-10-18 02:10:15	ID requested
⏵ 1	2022-03-04 10:46:04	announced

Dataset with its publication pending

submitter keyword: Localizations, Interactions, spatial proteomics

Dr Manuel Leonetti
contact affiliation	Group Leader, Cell Atlas
contact email	manuel.leonetti@czbiohub.org
lab head
Frank McCarthy
contact affiliation	CZ Biohub
contact email	frank.mccarthy@czbiohub.org
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/03/PXD029191

PRIDE project URI

• PRIDE
  1. PXD029191
     1. Label: PRIDE project
     2. Name: OpenCell: proteome-scale endogenous tagging enables the cartography of human cellular organization