PXD029167-1

PXD029167 is an original dataset announced via ProteomeXchange.

Title	Targeted mass spectrometry enables multiplexed quantification of immunomodulatory proteins in clinical biospecimens
Description	Immunotherapies are revolutionizing cancer care, producing durable responses and potentially cures in a subset of patients. However, response rates are low for most tumors, grade 3/4 toxicities are not uncommon, and our current understanding of tumor immunobiology is incomplete. While hundreds of immunomodulatory proteins in the tumor microenvironment shape the anti-tumor response, few of them can be reliably quantified. To address this need, we developed a multiplex panel of targeted proteomic assays to 52 peptides representing 46 proteins using peptide immunoaffinity enrichment coupled to multiple reaction monitoring-mass spectrometry. We validated the assays in tissue and plasma matrices, where performance figures of merit showed over 3 orders of dynamic range and median inter-day CVs of 5.2% (tissue) and 21% (plasma). A feasibility study in clinical biospecimens showed detection of 48/52 peptides in frozen tissue and 38/52 peptides in plasma. The assays are publicly available as a resource for the research community.
HostingRepository	PanoramaPublic
AnnounceDate	2021-11-08
AnnouncementXML	Submission_2021-11-08_07:36:21.177.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Jeff Whiteaker
SpeciesList	scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationList	Carbamidomethyl; Phospho; Label:13C(6); Label:13C(6)15N(2); Label:13C(6)15N(4)
Instrument	QTRAP 5500

Revision	Datetime	Status	ChangeLog Entry
0	2021-10-16 07:44:31	ID requested
⏵ 1	2021-11-08 07:36:21	announced
2	2022-02-28 17:01:23	announced	: Added PubMed Id

Jeffrey R. Whiteaker, Rachel A. Lundeen, Lei Zhao, Regine M. Schoenherr, Aura Burian, Dongqing Huang, Ulianna Voytovich, Tao Wang, Jacob J. Kennedy, Richard G. Ivey, Chenwei Lin, Oscar D. Murillo, Travis D. Lorentzen, Mathangi Thiagarajan, Simona Colantonio, Tessa W. Caceres, Rhonda R. Roberts, Joseph G. Knotts, Joshua J. Reading, Jan A. Kaczmarczyk, Christopher W. Richardson, Sandra S. Garcia-Buntley, William Bocik, Stephen M. Hewitt, Karen E. Murray, Nhan Do, Mary Brophy, Stephen W. Wilz, Hongbo Yu, Samuel Ajjarapu, Emily Boja, Tara Hiltke, Henry Rodriguez, Amanda G. Paulovich. Targeted mass spectrometry enables multiplexed quantification of immunomodulatory proteins in clinical biospecimens. Frontiers in Immunology, 2021, doi: 10.3389/fimmu.2021.765898.

submitter keyword: immuno-MRM, peptide immunoaffinity enrichment, immuno-oncology, immunotherapeutics, cancer

Jeff Whiteaker
contact affiliation	Fred Hutchinson Cancer Research Center
contact email	jwhiteak@fhcrc.org
lab head
Jeff Whiteaker
contact affiliation	Fred Hutchinson Cancer Research Center
contact email	jwhiteak@fhcrc.org
dataset submitter

Panorama Public dataset URI