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PXD029167 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleTargeted mass spectrometry enables multiplexed quantification of immunomodulatory proteins in clinical biospecimens
DescriptionImmunotherapies are revolutionizing cancer care, producing durable responses and potentially cures in a subset of patients. However, response rates are low for most tumors, grade 3/4 toxicities are not uncommon, and our current understanding of tumor immunobiology is incomplete. While hundreds of immunomodulatory proteins in the tumor microenvironment shape the anti-tumor response, few of them can be reliably quantified. To address this need, we developed a multiplex panel of targeted proteomic assays to 52 peptides representing 46 proteins using peptide immunoaffinity enrichment coupled to multiple reaction monitoring-mass spectrometry. We validated the assays in tissue and plasma matrices, where performance figures of merit showed over 3 orders of dynamic range and median inter-day CVs of 5.2% (tissue) and 21% (plasma). A feasibility study in clinical biospecimens showed detection of 48/52 peptides in frozen tissue and 38/52 peptides in plasma. The assays are publicly available as a resource for the research community.
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterJeff Whiteaker
SpeciesList scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationListCarbamidomethyl; Phospho; Label:13C(6); Label:13C(6)15N(2); Label:13C(6)15N(4)
InstrumentQTRAP 5500
Dataset History
RevisionDatetimeStatusChangeLog Entry
02021-10-16 07:44:31ID requested
12021-11-08 07:36:21announced
22022-02-28 17:01:23announced: Added PubMed Id
Publication List
Whiteaker JR, Lundeen RA, Zhao L, Schoenherr RM, Burian A, Huang D, Voytovich U, Wang T, Kennedy JJ, Ivey RG, Lin C, Murillo OD, Lorentzen TD, Thiagarajan M, Colantonio S, Caceres TW, Roberts RR, Knotts JG, Reading JJ, Kaczmarczyk JA, Richardson CW, Garcia-Buntley SS, Bocik W, Hewitt SM, Murray KE, Do N, Brophy M, Wilz SW, Yu H, Ajjarapu S, Boja E, Hiltke T, Rodriguez H, Paulovich AG, Targeted Mass Spectrometry Enables Multiplexed Quantification of Immunomodulatory Proteins in Clinical Biospecimens. Front Immunol, 12():765898(2021) [pubmed]
Keyword List
submitter keyword: immuno-MRM, peptide immunoaffinity enrichment, immuno-oncology, immunotherapeutics, cancer
Contact List
Jeff Whiteaker
contact affiliationFred Hutchinson Cancer Research Center
contact emailjwhiteak@fhcrc.org
lab head
Jeff Whiteaker
contact affiliationFred Hutchinson Cancer Research Center
contact emailjwhiteak@fhcrc.org
dataset submitter
Full Dataset Link List
Panorama Public dataset URI