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PXD029163-1

PXD029163 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleSPHINGOSINE 1-PHOSPHATE MEDIATES ADIPONECTIN RECEPTOR SIGNALING ESSENTIAL FOR LIPID HOMEOSTASIS AND EMBRYOGENESIS
DescriptionCells and organisms require proper membrane composition to function and develop. Phospholipids are the major component of membranes and are primarily acquired through the diet. Given great variability in diet composition, cells must be able to deploy mechanisms that correct deviations from optimal membrane composition and properties. Here, using unbiased lipidomics and proteomics, we found that the embryonic lethality in mice lacking the fluidity regulators Adiponectin Receptors 1 and 2 (AdipoR1/2) is associated with aberrant high saturation of the membrane phospholipids. Using mouse embryonic fibroblasts (MEFs) derived from AdipoR1/2-KO embryos, human cell lines and the model organism C. elegans we found that, mechanistically, AdipoR1/2-derived sphingosine 1-phosphate (S1P) signals in parallel through S1PR3-SREBP1 and PPARγ to sustain the expression of the fatty acid desaturase SCD and maintain membrane properties. Thus, our work identifies an evolutionary conserved pathway by which cells and organism maintain membrane homeostasis and adapt to a variable environment.
HostingRepositoryPRIDE
AnnounceDate2022-12-13
AnnouncementXMLSubmission_2022-12-13_06:56:15.118.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterProteomicsCore Facility
SpeciesList scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationListTMT6plex-126 reporter+balance reagent acylated residue; monohydroxylated residue
InstrumentOrbitrap Fusion
Dataset History
RevisionDatetimeStatusChangeLog Entry
02021-10-15 06:58:55ID requested
12022-12-13 06:56:15announced
22023-11-14 08:53:55announced2023-11-14: Updated project metadata.
Publication List
Keyword List
submitter keyword: PPARG, Laurdan, Embryonic Lethal, Palmitic acid,Tandem mass tag, TMT Adiponectin Receptor, SREBP, Membrane Fluidity, Phospholipid, SCD, Sphingosine 1-phosphate
Contact List
MarcPilon
contact affiliationProfessor, Department of Chemistry & Molecular Biology, University of Gothenburg, Box 462, 40530 Göteborg, Sweden
contact emailmarc.pilon@cmb.gu.se
lab head
ProteomicsCore Facility
contact affiliationSAMBIO Core Facilities, Sahgrenska Academy, University of Gothenburg
contact emailgupcf@outlook.com
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
Repository Record List
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