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PXD029156-1

PXD029156 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleCLUH controls astrin-1 expression to couple mitochondrial metabolism to cell cycle progression
DescriptionProliferating cells undergo metabolic changes in synchrony with cell cycle progression and cell division. Mitochondria provide fuel, metabolites, and ATP during different phases of the cell cycle, however it is not completely understood how mitochondrial function and the cell cycle are coordinated. CLUH is a post-transcriptional regulator of mRNAs encoding mitochondrial proteins involved in oxidative phosphorylation and several metabolic pathways. Here, we show a role of CLUH in regulating the expression of astrin, which is involved in metaphase to anaphase progression, centrosome integrity, and mTORC1 inhibition. We find that CLUH binds both the SPAG5 mRNA and its product astrin, and controls the synthesis and the stability of the full-length astrin-1 isoform. We show that CLUH interacts with astrin-1 specifically during interphase. Astrin-depleted cells show mislocalized CLUH at focal adhesions, mTORC1 hyperactivation and enhanced anabolism. On the other hand, cells lacking CLUH show decreased astrin levels and increased mTORC1 signaling, but cannot sustain anaplerotic and anabolic pathways. In absence of CLUH, cells fail to grow during G1, and progress faster through the cell cycle, indicating dysregulated matching of growth, metabolism and cell cycling. Our data reveal a role of CLUH in coupling growth signaling pathways and mitochondrial metabolism with cell cycle progression.
HostingRepositoryPRIDE
AnnounceDate2022-05-30
AnnouncementXMLSubmission_2022-05-29_23:58:38.871.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterPrerana Wagle
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListNo PTMs are included in the dataset
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02021-10-15 06:53:53ID requested
12022-05-29 23:58:39announced
22024-10-22 05:37:10announced2024-10-22: Updated project metadata.
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: CLUH
SPAG5
mitochondria
Contact List
Elena I. Rugarli
contact affiliationUniversity of Cologne
contact emailelena.rugarli@uni-koeln.de
lab head
Prerana Wagle
contact affiliationCECAD Research Center
contact emailproteomics-facility@uni-koeln.de
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
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