PXD029144-1

PXD029144 is an original dataset announced via ProteomeXchange.

Title	Proteomics and immunoproteomics of Anoplocephala perfoliata
Description	Anoplocephala perfoliata is a common tapeworm in horse with an ability to cause colic and increase mortality. At present, current diagnostic methods are not sensitive enough to detect A. perfoliata and immunoreactive excretory/secretory (E/S) proteins produced by this parasite, which comprise the most promising candidates for diagnostic tests. Here, we compared the E/S proteins produced by A. perfoliata worms of two different physiological states (small- and large-sized worms) in vitro by label-free quantitative (LFQ) proteomics with protein database composed of proteins encoded by Hymenolepis diminuta, Echinococcus multilocularis/granulosus and Taenia aseatica for protein identifications. The quantitative proteomics data was complemented with one-dimensional gel electrophoresis combined with immunoblotting using antisera from horses diagnosed positive and negative for this tapeworm. Protein bands cross-reacting most efficiently with the anti-A. perfoliata antibodies were subjected to in-gel digestion with LC-MS/MS identification. Both the non-gel and gel-based proteomic results were compared to propose a list of candidate proteins for further studies. These proteins will be validated for their suitability as diagnostic markers specific to cestodes and A. perfoliata.
HostingRepository	PRIDE
AnnounceDate	2022-11-11
AnnouncementXML	Submission_2022-11-11_00:32:26.038.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	TuulaNyman
SpeciesList	scientific name: Anoplocephala perfoliata; NCBI TaxID: 218192;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2021-10-15 03:22:05	ID requested
⏵ 1	2022-11-11 00:32:26	announced

Dataset with its publication pending

submitter keyword: Perseus, E/S proteins, Tapeworm,Parasite, Horse, MaxQuant, LC-MS/MS, 1D immunoblotting

TuulaNyman
contact affiliation	Head of Proteomics
contact email	tuula.nyman@medisin.uio.no
lab head
TuulaNyman
contact affiliation	University of Oslo
contact email	tuula.nyman@medisin.uio.no
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD029144
     1. Label: PRIDE project
     2. Name: Proteomics and immunoproteomics of Anoplocephala perfoliata