PXD029121 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Investigation of hepatic stellate cell proteomics during activa-tion with fetal bovine serum |
Description | Hepatic stellate cells (HSCs) are the major driving factor in liver fibrosis. Upon liver inflammation caused by alcohol abuse and/or a fatty liver, HSCs transform from a quiescent- into a proliferating, fibrotic phenotype. We study this transformation termed “activation”, using state of the art TIMS-TOF mass spectrometry proteomics, as well as phenotype analysis of the immortalized LX-2 HSC cell line. In our work we employ a simple, yet reliable model of HSC activation via an in-crease in growth media serum concentration (serum activation). Protein network analysis of ac-tivated LX 2 cells reveals an increase in the production of ribosomal proteins and proteins related to migration. Interestingly, we could also observe a decrease in the expression of lipogenic pro-teins and a loss of cytosolic lipid droplets during activation. Especially the downregulation of proteins associated with cholesterol biosynthesis might be a promising target for further study. This work provides an update on HSC activation characteristics using contemporary proteomic and bioinformatic analysis and presents an accessible model for HSC activation. |
HostingRepository | PRIDE |
AnnounceDate | 2022-02-15 |
AnnouncementXML | Submission_2022-02-15_11:02:27.720.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Maximilian Schinagl |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | timsTOF Pro |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2021-10-13 23:05:22 | ID requested | |
⏵ 1 | 2022-02-15 11:02:28 | announced | |
Publication List
Schinagl M, Tomin T, Gindlhuber J, Honeder S, Pfleger R, Schittmayer M, Trauner M, Birner-Gruenberger R, Proteomic Changes of Activated Hepatic Stellate Cells. Int J Mol Sci, 22(23):(2021) [pubmed] |
Keyword List
submitter keyword: Human, Liver, hepatic stellate cells, activation, fibrosis, proteomics |
Contact List
Ruth Birner-Gruenberger |
contact affiliation | Vienna University of Technology Institute of Chemical Technologies and Analytics Getreidemarkt 9/E164 A-1060 Vienna Austria |
contact email | ruth.birner-gruenberger@tuwien.ac.at |
lab head | |
Maximilian Schinagl |
contact affiliation | TU Vienna & Medical University of Graz |
contact email | maximilian.schinagl@tuwien.ac.at |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD029121
- Label: PRIDE project
- Name: Investigation of hepatic stellate cell proteomics during activa-tion with fetal bovine serum