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PXD029121-1

PXD029121 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleInvestigation of hepatic stellate cell proteomics during activa-tion with fetal bovine serum
DescriptionHepatic stellate cells (HSCs) are the major driving factor in liver fibrosis. Upon liver inflammation caused by alcohol abuse and/or a fatty liver, HSCs transform from a quiescent- into a proliferating, fibrotic phenotype. We study this transformation termed “activation”, using state of the art TIMS-TOF mass spectrometry proteomics, as well as phenotype analysis of the immortalized LX-2 HSC cell line. In our work we employ a simple, yet reliable model of HSC activation via an in-crease in growth media serum concentration (serum activation). Protein network analysis of ac-tivated LX 2 cells reveals an increase in the production of ribosomal proteins and proteins related to migration. Interestingly, we could also observe a decrease in the expression of lipogenic pro-teins and a loss of cytosolic lipid droplets during activation. Especially the downregulation of proteins associated with cholesterol biosynthesis might be a promising target for further study. This work provides an update on HSC activation characteristics using contemporary proteomic and bioinformatic analysis and presents an accessible model for HSC activation.
HostingRepositoryPRIDE
AnnounceDate2022-02-15
AnnouncementXMLSubmission_2022-02-15_11:02:27.720.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterMaximilian Schinagl
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListmonohydroxylated residue; iodoacetamide derivatized residue
InstrumenttimsTOF Pro
Dataset History
RevisionDatetimeStatusChangeLog Entry
02021-10-13 23:05:22ID requested
12022-02-15 11:02:28announced
Publication List
Schinagl M, Tomin T, Gindlhuber J, Honeder S, Pfleger R, Schittmayer M, Trauner M, Birner-Gruenberger R, Proteomic Changes of Activated Hepatic Stellate Cells. Int J Mol Sci, 22(23):(2021) [pubmed]
Keyword List
submitter keyword: Human, Liver, hepatic stellate cells, activation, fibrosis, proteomics
Contact List
Ruth Birner-Gruenberger
contact affiliationVienna University of Technology Institute of Chemical Technologies and Analytics Getreidemarkt 9/E164 A-1060 Vienna Austria
contact emailruth.birner-gruenberger@tuwien.ac.at
lab head
Maximilian Schinagl
contact affiliationTU Vienna & Medical University of Graz
contact emailmaximilian.schinagl@tuwien.ac.at
dataset submitter
Full Dataset Link List
Dataset FTP location
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