PXD029121-1

PXD029121 is an original dataset announced via ProteomeXchange.

Title	Investigation of hepatic stellate cell proteomics during activa-tion with fetal bovine serum
Description	Hepatic stellate cells (HSCs) are the major driving factor in liver fibrosis. Upon liver inflammation caused by alcohol abuse and/or a fatty liver, HSCs transform from a quiescent- into a proliferating, fibrotic phenotype. We study this transformation termed “activation”, using state of the art TIMS-TOF mass spectrometry proteomics, as well as phenotype analysis of the immortalized LX-2 HSC cell line. In our work we employ a simple, yet reliable model of HSC activation via an in-crease in growth media serum concentration (serum activation). Protein network analysis of ac-tivated LX 2 cells reveals an increase in the production of ribosomal proteins and proteins related to migration. Interestingly, we could also observe a decrease in the expression of lipogenic pro-teins and a loss of cytosolic lipid droplets during activation. Especially the downregulation of proteins associated with cholesterol biosynthesis might be a promising target for further study. This work provides an update on HSC activation characteristics using contemporary proteomic and bioinformatic analysis and presents an accessible model for HSC activation.
HostingRepository	PRIDE
AnnounceDate	2022-02-15
AnnouncementXML	Submission_2022-02-15_11:02:27.720.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Maximilian Schinagl
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	timsTOF Pro

Revision	Datetime	Status	ChangeLog Entry
0	2021-10-13 23:05:22	ID requested
⏵ 1	2022-02-15 11:02:28	announced

Schinagl M, Tomin T, Gindlhuber J, Honeder S, Pfleger R, Schittmayer M, Trauner M, Birner-Gruenberger R, Proteomic Changes of Activated Hepatic Stellate Cells. Int J Mol Sci, 22(23):(2021) [pubmed]

submitter keyword: Human, Liver, hepatic stellate cells, activation, fibrosis, proteomics

Ruth Birner-Gruenberger
contact affiliation	Vienna University of Technology Institute of Chemical Technologies and Analytics Getreidemarkt 9/E164 A-1060 Vienna Austria
contact email	ruth.birner-gruenberger@tuwien.ac.at
lab head
Maximilian Schinagl
contact affiliation	TU Vienna & Medical University of Graz
contact email	maximilian.schinagl@tuwien.ac.at
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD029121
     1. Label: PRIDE project
     2. Name: Investigation of hepatic stellate cell proteomics during activa-tion with fetal bovine serum