PXD029113-1

PXD029113 is an original dataset announced via ProteomeXchange.

Title	Sequestosome 1 is part of the interaction network of VAPB
Description	VAPB (Vesicle-Associated-membrane Protein-associated proteins B) is a tail-anchored mem-brane protein of the endoplasmic reticulum that can also be detected at the inner nuclear mem-brane. As a component of many contact sites between the endoplasmic reticulum and other or-ganelles, VAPB is engaged in multiple protein interactions with a plethora of binding partners. A mutant version of VAPB, P56S-VAPB, that results from a single point mutation is involved in a familial form of amyotrophic lateral sclerosis (ALS8). We performed RAPIDS (rapamycin- and APEX-dependent identification of proteins by SILAC) to identify proteins that interact with or are in close proximity to P56S-VAPB. The mutation abrogates the interaction of VAPB with many known binding partners. Instead, Sequestosome 1 (SQSTM1) was identified as a major in-teraction/proximity partner, a protein that is known as an autophagic adapter. Remarkably, not only the mutant P56S-VAPB, but also the wild type protein interacts with SQSTM1, as shown by proximity ligation assays and co-immunoprecipiation experiments.
HostingRepository	PRIDE
AnnounceDate	2022-02-15
AnnouncementXML	Submission_2022-02-15_11:03:38.543.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Christof Lenz
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2021-10-13 22:37:46	ID requested
⏵ 1	2022-02-15 11:03:39	announced

James C, Lenz C, Urlaub H, Kehlenbach RH, Sequestosome 1 Is Part of the Interaction Network of VAPB. Int J Mol Sci, 22(24):(2021) [pubmed]

submitter keyword: VAPB

amyotrophic lateral sclerosis

SQSTM1

sequestosome, p62

Christof Lenz
contact affiliation	Core Facility Proteomics, Department of Clinical Chemistry, University Medical Center Göttingen, Germany
contact email	christof.lenz@med.uni-goettingen.de
lab head
Christof Lenz
contact affiliation	Max Planck Institute for Biophysical Chemistry
contact email	christof.lenz@mpibpc.mpg.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/02/PXD029113

PRIDE project URI

• PRIDE
  1. PXD029113
     1. Label: PRIDE project
     2. Name: Sequestosome 1 is part of the interaction network of VAPB