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PXD029090-1

PXD029090 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleMembrane vesicles of Pectobacterium as an underappreciated protein secretion system
DescriptionPectobacterium are Gram-negative rods of the family Pectobacteriaceae. They are the causative agent of soft rot diseases of crops and ornamental plants. However, their virulence mechanisms are not yet fully elucidated. Membrane vesicles (MVs) are universally released by bacteria and are be-lieved to play an important role in pathogenicity, and survival of bacteria in the environment. Our study investigates the role of MVs in the virulence of Pectobacterium. The results indicate that the morphology and yields of MVs depend on medium composition. In polygalacturonic acid (PGA) supplemented media, Pectobacterium produce MVs of a larger size (100-300 nm) apart of vesicles below 100 nm. Proteomic analyses revealed the presence of pectate degrading enzymes in MVs. The pectate plate test and enzymatic assay proved that those enzymes are active and able to de-grade pectates. What is more, pathogenicity test indicated that MVs derived from Pectobacterium were able to induce maceration of Zantedeschia sp. leaves. We also show that MVs of β-lactamase producing strains were able to suppress ampicillin activity and permit the growth of susceptible bacteria. Those findings indicate that MVs of Pectobacterium play an important role in host-pathogen interactions and niche competition with other bacteria. Our research also sheds some light on the mechanism of MVs production. We demonstrate that Pectobacterium strains, which overexpress the green fluorescence protein (GFP), produce more MVs than wild type strains. Moreover, proteomic analysis revealed that GFP was present in MVs. Therefore, we demonstrate that protein sequestration into MVs is not limited strictly to periplasmic proteins and is a common occurrence. Our research highlights the importance of MVs production as a mechanism of cargo delivery in Pectobacterium and an alternative secretion system.
HostingRepositoryPRIDE
AnnounceDate2022-02-15
AnnouncementXMLSubmission_2022-02-15_10:55:41.117.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterPaulina Czaplewska
SpeciesList scientific name: Pectobacterium zantedeschiae; NCBI TaxID: 2034769;
ModificationListmonohydroxylated residue; iodoacetamide derivatized residue
InstrumentTripleTOF 5600
Dataset History
RevisionDatetimeStatusChangeLog Entry
02021-10-12 08:13:32ID requested
12022-02-15 10:55:42announced
Publication List
Jonca J, Waleron M, Czaplewska P, Bogucka A, Ste, ć A, Dziomba S, Jasiecki J, Rych, ł, owski M, Waleron K, as an Effective Protein Secretion System. Int J Mol Sci, 22(22):(2021) [pubmed]
Keyword List
submitter keyword: bacterial membrane vesicles
Pectobacterium
proteomic analysis
plant pathogens
bacterial virulence
Contact List
Paulina Czaplewska
contact affiliationUniversuty of Gdansk, Intercollegiate Faculty of Biotechnology, Laboratory of Mass Spectrometry
contact emailpaulina.czaplewska@ug.edu.pl
lab head
Paulina Czaplewska
contact affiliationUniversity of Gdansk
contact emailpaulina.czaplewska@ug.edu.pl
dataset submitter
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Dataset FTP location
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