PXD029090-1

PXD029090 is an original dataset announced via ProteomeXchange.

Title	Membrane vesicles of Pectobacterium as an underappreciated protein secretion system
Description	Pectobacterium are Gram-negative rods of the family Pectobacteriaceae. They are the causative agent of soft rot diseases of crops and ornamental plants. However, their virulence mechanisms are not yet fully elucidated. Membrane vesicles (MVs) are universally released by bacteria and are be-lieved to play an important role in pathogenicity, and survival of bacteria in the environment. Our study investigates the role of MVs in the virulence of Pectobacterium. The results indicate that the morphology and yields of MVs depend on medium composition. In polygalacturonic acid (PGA) supplemented media, Pectobacterium produce MVs of a larger size (100-300 nm) apart of vesicles below 100 nm. Proteomic analyses revealed the presence of pectate degrading enzymes in MVs. The pectate plate test and enzymatic assay proved that those enzymes are active and able to de-grade pectates. What is more, pathogenicity test indicated that MVs derived from Pectobacterium were able to induce maceration of Zantedeschia sp. leaves. We also show that MVs of β-lactamase producing strains were able to suppress ampicillin activity and permit the growth of susceptible bacteria. Those findings indicate that MVs of Pectobacterium play an important role in host-pathogen interactions and niche competition with other bacteria. Our research also sheds some light on the mechanism of MVs production. We demonstrate that Pectobacterium strains, which overexpress the green fluorescence protein (GFP), produce more MVs than wild type strains. Moreover, proteomic analysis revealed that GFP was present in MVs. Therefore, we demonstrate that protein sequestration into MVs is not limited strictly to periplasmic proteins and is a common occurrence. Our research highlights the importance of MVs production as a mechanism of cargo delivery in Pectobacterium and an alternative secretion system.
HostingRepository	PRIDE
AnnounceDate	2022-02-15
AnnouncementXML	Submission_2022-02-15_10:55:41.117.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Paulina Czaplewska
SpeciesList	scientific name: Pectobacterium zantedeschiae; NCBI TaxID: 2034769;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	TripleTOF 5600

Revision	Datetime	Status	ChangeLog Entry
0	2021-10-12 08:13:32	ID requested
⏵ 1	2022-02-15 10:55:42	announced

Jonca J, Waleron M, Czaplewska P, Bogucka A, Ste, ć A, Dziomba S, Jasiecki J, Rych, ł, owski M, Waleron K, as an Effective Protein Secretion System. Int J Mol Sci, 22(22):(2021) [pubmed]

submitter keyword: bacterial membrane vesicles

Pectobacterium

proteomic analysis

plant pathogens

bacterial virulence

Paulina Czaplewska
contact affiliation	Universuty of Gdansk, Intercollegiate Faculty of Biotechnology, Laboratory of Mass Spectrometry
contact email	paulina.czaplewska@ug.edu.pl
lab head
Paulina Czaplewska
contact affiliation	University of Gdansk
contact email	paulina.czaplewska@ug.edu.pl
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD029090
     1. Label: PRIDE project
     2. Name: Membrane vesicles of Pectobacterium as an underappreciated protein secretion system