PXD029076-1

PXD029076 is an original dataset announced via ProteomeXchange.

Title	Antioxidant, Immunomodulatory and Potential Anticancer Capacity of Polysaccharides (Glucans) from Euglena gracilis G.A. Klebs
Description	The present study was carried out to determine the bioactivity of polysaccharides extracted from Euglena gracilis (EgPs). These were characterized by FT-IR and GC-MS. Cytotoxicity analyses (MTT) were performed on healthy human gingival fibroblast cell lines (HGF-1), obtaining an IC50 of 228.66 µg mL-1, and cell lines with anticancer activity for colon cancer (HCT-116), breast cancer (MCF-7), human leukemia (U-937, HL-60) and lung cancer (NCl-H460), showing that EgPs have anticancer activity, mainly in HTC-116 cells (IC50 = 26.1 µg mL-1). The immunological assay determined the immunomodulatory capacity of polysaccharides for the production of proinflammatory cytokines IL-6 and TNF-α in murine macrophages (RAW 264.7) and TNF-α in human monocytes (THP-1). It was observed that the EgPs had a stimulating capacity in the synthesis of these interleukins. The antioxidant capacity of polysaccharides and their biomass were analyzed using the ABTS method (18.30 ± 0.14% and (5.40 ± 0.56%, respectively, and the DPPH method for biomass (17.79 ± 0.57%). We quantitatively profiled HGF-1 proteins by liquid chromatography-tandem mass spectrometry analysis, coupled with 2-plex tandem mass tag labelling, in normal cells. In total, 1346 proteins were identified and quantified with high confidence, of which five were considered to be overexpressed. The data is available through ProteomeXchange, under identifier PXD029076.
HostingRepository	PRIDE
AnnounceDate	2022-12-21
AnnouncementXML	Submission_2022-12-21_05:02:53.260.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	CasimiroCardenas
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2021-10-12 07:52:33	ID requested
⏵ 1	2022-12-21 05:02:53	announced
2	2023-11-14 08:53:38	announced	2023-11-14: Updated project metadata.

submitter keyword: TMT-2plex., sulfated polysaccharides, anticancer capacity, nutraceuticals,Euglena gracilis, immunomodulatory capacity, antioxidant activity

Roberto AbdalaDiaz
contact affiliation	University of Malaga, Faculty of Sciences, Department of Ecology, Malaga, Spain.
contact email	abdala@uma.es
lab head
CasimiroCardenas
contact affiliation	University of Malaga
contact email	ccg@uma.es
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD029076
     1. Label: PRIDE project
     2. Name: Antioxidant, Immunomodulatory and Potential Anticancer Capacity of Polysaccharides (Glucans) from Euglena gracilis G.A. Klebs