PXD029070-1

PXD029070 is an original dataset announced via ProteomeXchange.

Title	Rat vascular smooth muscle cells LC-MS/MS
Description	Crotonylation of histones is discovered of late as one of post-translational modification that can regulate gene expression. However, the function of crotonylation on non-histone proteins in vascular smooth muscle cells (VSMC) is unclear. Here, we aim to use modification and proteomic analysis to find the cellular characteristic of crotonylated non-histone proteins and the crosstalk with ubiquitinated proteins in vascular smooth muscle cell (VSMC) phenotypic remodeling. We performed modification and proteomic analysis of VSMCs before and after stimulated with platelet-derived growth factor-BB (PDGF-BB). The crotonylated and ubiquitinated pan-antibody was used to enrich the protein and then subjected to high-throughput mass spectrometry analysis. The enrichment analysis was performed within differentially modified proteins in regards to GO terms, KEGG and protein domain.
HostingRepository	PRIDE
AnnounceDate	2022-05-19
AnnouncementXML	Submission_2022-05-19_03:43:10.087.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Li-Hua Dong
SpeciesList	scientific name: Rattus norvegicus (Rat); NCBI TaxID: 10116;
ModificationList	ubiquitinylated lysine; crotonylated L-lysine
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2021-10-11 22:11:10	ID requested
⏵ 1	2022-05-19 03:43:11	announced

Cao SH, Chen ZH, Ma RY, Yue L, Jiang HM, Dong LH, Dynamics and Functional Interplay of Nonhistone Lysine Crotonylome and Ubiquitylome in Vascular Smooth Muscle Cell Phenotypic Remodeling. Front Cardiovasc Med, 9():783739(2022) [pubmed]

submitter keyword: VSMC, LC-MS/MS

Li-Hua Dong
contact affiliation	Department of Biochemistry and Molecular Biology, College of Basic Medicine, Key Laboratory of Medical Biotechnology of Hebei Province, Hebei Medical University, Shijiazhuang, China
contact email	donglihua@hebmu.edu.cn
lab head
Li-Hua Dong
contact affiliation	Hebei Medical University
contact email	donglihua@hebmu.edu.cn
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD029070
     1. Label: PRIDE project
     2. Name: Rat vascular smooth muscle cells LC-MS/MS