PXD029069-1

PXD029069 is an original dataset announced via ProteomeXchange.

Title	Metallothionein immunohistochemistry has high sensitivity and specificity for detection of Wilson disease
Description	Diagnosis of Wilson disease (WD) can be difficult because of its protean clinical presentation, but early diagnosis is important because effective treatment is available and can prevent disease progression. Using quantitative proteomics, we characterized proteins that are differentially expressed in WD compared to normal liver. Further the expression of MT1M and COX17 were validated using immunohistochemistry. We found diffuse MT immunoreactivity in all liver specimens from patients with WD (n=20), the intensity of the staining was moderate to strong. This staining pattern was distinct from that seen in specimens from the control groups which included diseases that may be in the clinical or histologic differential of WD (steatohepatitis (n=51), chronic viral hepatitis (n=40), autoimmune hepatitis (n=50), chronic biliary tract disease (n=42), and normal liver (n=20)). COX17 immunostain showed no significant difference in expression between the WD and control groups. MT had higher sensitivity than rhodanine for diagnosis of WD. While the quantitative liver copper assays also had high sensitivity, they require more tissue, have a higher cost, have longer turnaround time, and are less widely available than an immunohistochemical stain. We conclude that MT IHC is a sensitive immunohistochemical stain for the diagnosis of Wilson disease that could be widely deployed as a screening tool for liver biopsies in which Wilson disease is in the clinical or histologic differential diagnosis.
HostingRepository	PRIDE
AnnounceDate	2023-04-18
AnnouncementXML	Submission_2023-04-18_09:46:37.499.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	AkhileshPandey
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Eclipse

Revision	Datetime	Status	ChangeLog Entry
0	2021-10-11 22:10:44	ID requested
⏵ 1	2023-04-18 09:46:39	announced
2	2023-11-14 08:44:58	announced	2023-11-14: Updated project metadata.

Rowan DJ, Mangalaparthi KK, Singh S, Moreira RK, Mounajjed T, Lamps L, Westerhoff M, Cheng J, Bellizzi AM, Allende DS, Pandey A, Graham RP, Metallothionein immunohistochemistry has high sensitivity and specificity for detection of Wilson disease. Mod Pathol, 35(7):946-955(2022) [pubmed]

submitter keyword: Quantitative proteomics, Immunohistochemistry, liver, Wilson disease, MT1G, Sensitivity,Metallothionein-1G, Specificity

AkhileshPandey
contact affiliation	Department of Laboratory Medicine and Pathology, Mayo Clinic, 200 First ST SW, Rochester, MN 55905, USA
contact email	pandey.akhilesh@mayo.edu
lab head
AkhileshPandey
contact affiliation	Department of Laboratory Medicine and Pathology, Mayo Clinic, Rochester, MN 55905
contact email	pandey.akhilesh@mayo.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/04/PXD029069

PRIDE project URI

• PRIDE
  1. PXD029069
     1. Label: PRIDE project
     2. Name: Metallothionein immunohistochemistry has high sensitivity and specificity for detection of Wilson disease