PXD029052-1

PXD029052 is an original dataset announced via ProteomeXchange.

Title	Proteomics profiling to distinguish DOCK8 deficiency from atopic dermatitis
Description	Background: DOCK8 deficiency is an autosomal recessive form of hyperimmunoglobulinemia E syndrome (HIES). Severe atopic dermatitis (AD) shares with DOCK8 deficiency some clinical symptoms, including eczema, eosinophilia, and increased serum IgE levels. The deficiency of DOCK8 protein is potentially a life-threatening autosomal recessive HIES and only curable with bone marrow transplantation. Despite identified metabolomics and cytokine biomarkers, novel proteomics biomarkers need to be identified, as the connecting networks are critical to our understanding of this disease. Hence we performed serum proteomics profiling using LC-MSE SynaptG2. Method: Label-free untargeted proteomics analysis was used to identify potentially reliable, sensitive, and specific protein biomarkers in serum collected from DOCK8 (n=10), AD (n=9) patients, which were compared to ctrls (n=5). Results: From a total of 275 quantifiable proteins, binary comparisons between AD vs. Ctrl, DOCK8 vs. Ctrl, and DOCK8 vs. AD revealed 109, 105 and 85 dysregulated proteins, respectively. 24 among 85 proteins were specific potential biomarkers among the DOCK8 and AD groups. The sensitivity and specificity of few proteins like Claspin, Immunoglobulin kappa and heavy, complement components as potential biomarkers to distinguish between DOCK8 and AD patients were evaluated using the receiver operating characteristic curve. DOCK8 deficiency and AD groups' profiling revealed a shared role of ERK1/2 among the commonly dysregulated proteins. Conclusion: In this study, we have identified potential proteomics biomarkers and profile to distinguish between DOCK8 and AD, with possible diagnostic and therapeutic applications to help create effective interventions for managing these diseases. Further studies to confirm these associations in prospective cohorts are warranted.
HostingRepository	PRIDE
AnnounceDate	2022-05-17
AnnouncementXML	Submission_2022-05-17_06:55:34.534.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Ayodele Alaiya
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue; deamidated residue
Instrument	Synapt MS

Revision	Datetime	Status	ChangeLog Entry
0	2021-10-11 03:55:40	ID requested
⏵ 1	2022-05-17 06:55:35	announced
2	2024-10-22 05:36:12	announced	2024-10-22: Updated project metadata.

Jacob M, Masood A, Shinwari Z, Abdel Jabbar M, Al-Mousa H, Arnaout R, AlSaud B, Dasouki M, Alaiya AA, Abdel Rahman AM, Proteomics Profiling to Distinguish DOCK8 Deficiency From Atopic Dermatitis. Front Allergy, 2():774902(2021) [pubmed]

submitter keyword: Atopic dermatitis, Dedicator of Cytokinesis (DOCK8), Hyper IgE Syndrome (HIES), label-free Proteomics, LC-MSE SynaptG2, Multiple-Reaction Monitoring

Dr. Ayodele Alaiya
contact affiliation	King faisal Specialist Hospital and esearch Center
contact email	aalaiya@kfshrc.edu.sa
lab head
Ayodele Alaiya
contact affiliation	Therapeutics & Biomarker Discovery for Clinical Applications, Stem Cell Program, King Faisal Specialist Hospital and Research Center, Riyadh, Saudi Arabia
contact email	aalaiya@kfshrc.edu.sa
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD029052
     1. Label: PRIDE project
     2. Name: Proteomics profiling to distinguish DOCK8 deficiency from atopic dermatitis