PXD029047-1

PXD029047 is an original dataset announced via ProteomeXchange.

Title	Multilayered Regulation of Autophagy by the Atg1 Kinase Orchestrates Spatial and Temporal Control of Autophagosome Formation
Description	Autophagy is a conserved intracellular degradation pathway exerting various cytoprotective and homeostatic functions by utilizing de novo double membrane vesicle (autophagosome) formation to target a wide range of cytoplasmic material for vacuolar/lysosomal degradation. The Atg1 kinase is one of its key regulators coordinating a complex signaling program to orchestrate autophagosome formation. Combining in vitro reconstitution and cell based approaches, we demonstrate that Atg1 is activated by lipidated Atg8 (Atg8-PE) stimulating substrate phosphorylation along the growing autophagosomal membrane. Atg1 dependent phosphorylation of Atg13 triggers Atg1 complex dissociation resulting in rapid turnover of Atg1 complex subunits at the pre-autophagosomal structure. Moreover, Atg1 recruitment by Atg8-PE self-regulates Atg8-PE levels in the growing autophagosomal membrane by phosphorylating and inhibiting the Atg8 specific E2 and E3. Taken together, our work uncovers the molecular basis for positive and negative feedback imposed by Atg1, and opposing phosphorylation and dephosphorylation events governing the spatiotemporal regulation of autophagy.
HostingRepository	PRIDE
AnnounceDate	2021-10-11
AnnouncementXML	Submission_2021-10-11_13:57:39.113.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Ben Collins
SpeciesList	scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
ModificationList	phosphorylated residue; acetylated residue; monohydroxylated residue
Instrument	timsTOF Pro; TripleTOF 5600; LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2021-10-11 02:17:18	ID requested
⏵ 1	2021-10-11 13:57:39	announced

Dataset with its publication pending

submitter keyword: Autophagy, signalling, phosphorylation, protein kinases, protein phosphatases, ubiquitin-like proteins, Atg8 lipidation, metabolism

Ben Collins
contact affiliation	Queen's University Belfast
contact email	ben.collins@qub.ac.uk
lab head
Ben Collins
contact affiliation	Queen's University Belfast
contact email	ben.collins@qub.ac.uk
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD029047
     1. Label: PRIDE project
     2. Name: Multilayered Regulation of Autophagy by the Atg1 Kinase Orchestrates Spatial and Temporal Control of Autophagosome Formation