PXD029025-1

PXD029025 is an original dataset announced via ProteomeXchange.

Title	The membrane proteome of spores and vegetative cells of the food-borne pathogen Bacillus cereus.
Description	Membrane proteins are fascinating since they play an important role in diverse cellular functions and constitute many drug targets. Membrane proteins are challenging to analyze. The spore, the most resistant form of life known, harbors a compressed inner membrane. This membrane acts not only as a barrier for undesired molecules but also as a scaffold for proteins involved in signal transduction and the transport of metabolites during spore germination and subsequent vegetative growth. In this study, we adapted a membrane enrichment method to study the membrane proteome of spores and cells of the food-borne pathogen Bacillus cereus using quantitative proteomics. Using bioinformatics filtering we identify and quantify 498 vegetative cell membrane proteins and 244 spore inner membrane proteins. Comparison of vegetative and spore membrane proteins showed there were 54 spore membrane specific and 308 cell membrane specific proteins. Functional characterization of these proteins showed that the cell membrane proteome has a far larger number of transporters, receptors and proteins related to cell division and motility. This was also reflected in the much higher expression level of many of these proteins in the cellular membrane for those proteins that were in common with the spore inner membrane. The spore inner membrane had specific expression of several germinant receptor and spore specific proteins, but also seemed to show a preference towards the use of simple carbohydrates like glucose and fructose owing to only expressing transporters for these. These results show the differences in membrane proteome composition and show us the specific proteins necessary in the inner membrane of a dormant spore of this toxigenic spore forming bacterium to survive adverse conditions.
HostingRepository	MassIVE
AnnounceDate	2022-10-14
AnnouncementXML	Submission_2022-10-14_02:13:51.240.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Gertjan Kramer
SpeciesList	scientific name: Bacillus cereus; NCBI TaxID: 1396;
ModificationList	Carbamidomethyl; Oxidation
Instrument	timsTOF Pro

Revision	Datetime	Status	ChangeLog Entry
0	2021-10-10 06:17:51	ID requested
⏵ 1	2022-10-14 02:13:51	announced

Xiaowei Gao ,Bhagyashree N. Swarge, Henk L. Dekker, Winfried Roseboom, Stanley Brul, and Gertjan Kramer. The Membrane Proteome of Spores and Vegetative Cells of the Food-Borne Pathogen Bacillus cereus. Int. J. Mol. Sci. 2021, 22(22), 12475.

submitter keyword: Membrane Proteome, Bacillus cereus, Spores, Label Free Mass Spectrometry, Proteomics

Gertjan Kramer
contact affiliation	University of Amsterdam
contact email	gertjan.kramer@uva.nl
lab head
Gertjan Kramer
contact affiliation	Universiteit van Amsterdam
contact email	gertjan.kramer@uva.nl
dataset submitter

MassIVE dataset URI

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