PXD029012-2

PXD029012 is an original dataset announced via ProteomeXchange.

Title	The proteome of neuroglobin-overexpressing SH-SY5Y neuroblastoma cells
Description	Neuroglobin (NGB) is an oxygen-binding globin expressed in vertebrates mainly in the central and peripheral nervous system, but also in extra-neuronal cells. NGB is involved in oxygen transport, storage, and homeostasis. The role hypothesized for this globin is in fact related to neuroprotection against oxidative stress. Also, NGB is functionally and physically associated to mitochondria, possibly playing a role in ATP production and regulation. NGB actually exerts its function upon up-regulation. Thus, NGB-overexpressing SH-SY5Y neuroblastoma cells (NGB-FLAG) were analyzed by shotgun label-free quantitative proteomics in comparison with control cells (CTRL).
HostingRepository	PRIDE
AnnounceDate	2021-12-20
AnnouncementXML	Submission_2022-02-01_03:28:50.148.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Michele Costanzo
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	iodoacetamide derivatized residue
Instrument	LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2021-10-08 06:46:46	ID requested
1	2021-12-20 09:02:28	announced
⏵ 2	2022-02-01 03:28:50	announced	2022-02-01: Updated publication reference for PubMed record(s): 34943907.

Manganelli V, Salvatori I, Costanzo M, Capozzi A, Caissutti D, Caterino M, Valle C, Ferri A, Sorice M, Ruoppolo M, Garofalo T, Misasi R, Overexpression of Neuroglobin Promotes Energy Metabolism and Autophagy Induction in Human Neuroblastoma SH-SY5Y Cells. Cells, 10(12):(2021) [pubmed]

submitter keyword: Neuroglobin, NGB, label-free proteomics, S-Trap

Michele Costanzo
contact affiliation	Department of Molecular Medicine and Medical Biotechnology, University of Naples "Federico II", Italy
contact email	michele.costanzo@unina.it
lab head
Michele Costanzo
contact affiliation	Department of Molecular Medicine and Medical Biotechnology - University of Naples Federico II
contact email	michele.costanzo@unina.it
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/12/PXD029012

PRIDE project URI

• PRIDE
  1. PXD029012
     1. Label: PRIDE project
     2. Name: The proteome of neuroglobin-overexpressing SH-SY5Y neuroblastoma cells