PXD029003-1

PXD029003 is an original dataset announced via ProteomeXchange.

Title	Development of a novel minimally invasive sampling and analysis technique for bioarchaeological proteomics
Description	Here we present the development of a novel minimally invasive sample preparation technique for application in the mass spectrometric analysis of bioarchaeological materials. The extraction protocol was developed by applying commercially available, dermatology grade skin sampling strips to modern skin surfaces as a surrogate, and then applied to cranial and bone fragments belonging to a 26th Dynasty Egyptian mummified individual from the coffin of a woman named Mer-Neith-it-es. Extracted proteins were subjected to electrophoretic separation and proteolytic digestion, resulting in peptides that were separated, fragmented and identified using nanoflow liquid chromatography - high resolution tandem mass spectrometry. We have identified a number of ancient brain and intracellular proteins on the surfaces of the various cranial and bone fragments, without causing any significant damage to these valuable remains.
HostingRepository	PRIDE
AnnounceDate	2022-04-06
AnnouncementXML	Submission_2022-04-06_09:27:43.179.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Dylan Multari
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	hydroxylated proline; iodoacetamide derivatized residue; deamidated residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2021-10-08 06:40:52	ID requested
⏵ 1	2022-04-06 09:27:43	announced

Dataset with its publication pending

submitter keyword: bioarchaeology

human

proteomics

minimally invasive sampling

mass spectrometry

LC-MSMS

Paul Andrew Haynes
contact affiliation	Haynes Lab, Department of Molecular Sciences, Faculty of Science and Engineering, Macquarie University, Australia
contact email	paul.haynes@mq.edu.au
lab head
Dylan Multari
contact affiliation	Macquarie University
contact email	dylan.multari@hdr.mq.edu.au
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/04/PXD029003

PRIDE project URI

• PRIDE
  1. PXD029003
     1. Label: PRIDE project
     2. Name: Development of a novel minimally invasive sampling and analysis technique for bioarchaeological proteomics