PXD028997-1

PXD028997 is an original dataset announced via ProteomeXchange.

Title	Selected reaction monitoring based analysis of proteins in liver tissue of Labeo rohita
Description	Bottom-up proteomics analysis relies on efficient protein digestion for performing mass spectrometric analysis. Optimisation of protein solubilisation and digestion strategies is a key step for obtaining reliable proteomic data. In this study, two different solubilisation buffers (SDS and Urea) and three digestion methods (FASP, S-Trap and in-solution,) were applied for the proteomic analysis of liver tissue of Labeo rohita. Label free quantification analysis was performed to analyse the similarities and differences in the results obtained in each method. Based on the discovery data, few proteins were selected for targeted data acquisition. These proteins were chosen on the basis of highest peptide spectral matches in different experimental conditions. The aim was to validate the trends in abundance of proteins in different experimental conditions.
HostingRepository	PanoramaPublic
AnnounceDate	2022-08-01
AnnouncementXML	Submission_2022-08-01_14:06:41.485.xml
DigitalObjectIdentifier
ReviewLevel	Non peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Sanjeeva Srivastava
SpeciesList	scientific name: Labeo rohita; NCBI TaxID: 84645;
ModificationList	Carbamidomethyl; Label:13C(6)15N(2)
Instrument	TSQ Altis

Revision	Datetime	Status	ChangeLog Entry
0	2021-10-07 20:03:46	ID requested
⏵ 1	2022-08-01 14:06:41	announced
2	2023-03-22 14:24:19	announced	: Added PubMed Id

Dataset with its publication pending

submitter keyword: FASP, method optimization, rohu liver, SRM, STRAP

Sanjeeva Srivastava
contact affiliation	Indian Institute of Technology, Bombay
contact email	sanjeeva@iitb.ac.in
lab head
Sanjeeva Srivastava
contact affiliation	Indian Institute of Technology, Bombay
contact email	sanjeeva@iitb.ac.in
dataset submitter

Panorama Public dataset URI