PXD028973-1

PXD028973 is an original dataset announced via ProteomeXchange.

Title	The F-box protein Bard (CG14317) targets the Smaug RNA-binding protein for destruction during the Drosophila maternal-to-zygotic transition
Description	During the maternal-to-zygotic transition (MZT), which encompasses the earliest stages of animal embryogenesis, a subset of maternally supplied gene products is cleared, thus permitting activation of zygotic gene expression. In the Drosophila melanogaster embryo, the RNA-binding protein Smaug plays an essential role in progression through the MZT by regulating the translational repression and degradation of a large number of maternal mRNA species. The Smaug protein itself is rapidly cleared at the end of the MZT by the Skp/Cullin/F-box (SCF) E3-ligase complex; clearance of Smaug requires zygotic transcription. Here, we show that an F-box protein, which we name Bard (encoded by CG14317), is required for degradation of Smaug. Bard is expressed zygotically and physically interacts with Smaug at the end of the MZT, coincident with binding of the maternal SCF proteins, SkpA and Cullin1, and with degradation of Smaug. We show that shRNA-mediated knock-down of Bard or deletion of the bard gene in the early embryo results in stabilization of Smaug protein, a phenotype that is rescued by transgenes expressing Bard .
HostingRepository	MassIVE
AnnounceDate	2021-10-12
AnnouncementXML	Submission_2021-10-12_04:23:33.500.xml
DigitalObjectIdentifier
ReviewLevel	Non peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Sichun Lin
SpeciesList	scientific name: Drosophila melanogaster; common name: fruit fly; NCBI TaxID: 7227;
ModificationList	unknown modification
Instrument	instrument model

Revision	Datetime	Status	ChangeLog Entry
0	2021-10-06 20:11:41	ID requested
⏵ 1	2021-10-12 04:23:33	announced

no publication

submitter keyword: Smaug, RNA-binding protein, E3 ubiquitin ligase, Skp/Cullin/F-box (SCF) complex, Bard/CG14317

Howard Lipshitz
contact affiliation	University of Toronto
contact email	howard.lipshitz@utoronto.ca
lab head
Sichun Lin
contact affiliation	University of Toronto, Leslie Dan Faculty of Pharm
contact email	sichun.lin@utoronto.ca
dataset submitter

MassIVE dataset URI

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